Oxidatively modified low-density lipoprotein in mononuclear cells detected by laser-induced fluorescence spectroscopy

Tami N. Glenn; Alexander A. Oraevsky; Frank K. Tittel; Sharon L. Thomsen M.D.; Steven L. Jacques; Philip D. Henry

doi:10.1117/12.209122

12 May 1995 Oxidatively modified low-density lipoprotein in mononuclear cells detected by laser-induced fluorescence spectroscopy

Tami N. Glenn, Alexander A. Oraevsky, Frank K. Tittel, Sharon L. Thomsen M.D., Steven L. Jacques, Philip D. Henry

Author Affiliations +

Proceedings Volume 2395, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems V; (1995) https://doi.org/10.1117/12.209122
Event: Photonics West '95, 1995, San Jose, CA, United States

Abstract

Hyperlipidemic states are associated with focal accumulations in arterial walls of oxidatively modified low density lipoprotein (LDL) and monocyte-derived liquid-laden macrophages, biochemical and cellular hallmarks of atheromatous lesions. Mechanisms underlying the generation and cellular uptake of oxidized LDL are still incompletely understood. We have used laser-induced fluorescence spectroscopy to monitor the formation, intracellular accumulation, and tissue distribution of oxidized LDL. Oxidatively modified LDL excited by a XeCl excimer laser (308 nm) exhibits unique spectral characteristics that distinguish it from native (non-oxidized) LDL. The same spectral characteristics were demonstrated in lipid-rich atheromatous lesions, macrophages after incubation with oxidized LDL, and peripheral blood monocytes from hyperlipidemic, but not normolipidemic subjects. Detection of oxidized LDL in peripheral blood monocytes and arterial tissue may provide information on the atherogenic activity of hyperlipidemic states and serve as a novel risk factor for the assessment of atherosclerosis.

Citation Download Citation

Tami N. Glenn, Alexander A. Oraevsky, Frank K. Tittel, Sharon L. Thomsen M.D., Steven L. Jacques, and Philip D. Henry "Oxidatively modified low-density lipoprotein in mononuclear cells detected by laser-induced fluorescence spectroscopy", Proc. SPIE 2395, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems V, (12 May 1995); https://doi.org/10.1117/12.209122

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available