Photodynamic treatment of lens epithelial cells for cataract surgery

Robert W. Lingua M.D.; Jean-Marie A. Parel; Gabriel Simon; Kam Li

doi:10.1117/12.43962

1 June 1991 Photodynamic treatment of lens epithelial cells for cataract surgery

Robert W. Lingua M.D., Jean-Marie A. Parel, Gabriel Simon, Kam Li

Proceedings Volume 1423, Ophthalmic Technologies; (1991) https://doi.org/10.1117/12.43962
Event: Optics, Electro-Optics, and Laser Applications in Science and Engineering, 1991, Los Angeles, CA, United States

Abstract

Photodynamic therapy (PDT) eiiploying Dihematopor*iyrin ethers (DHE) (Photofrin II) at pharmacologic lvels, has been denonstrate3 to kill rabbit lens epithelial cells, in vivo. This in vitro study, reports on the minimal necessary parameters for rabbit lens epithelial cell death. Explants of rabbit lenses were incubated in various concentrations of DHE (1O,, 100, 500, 1000 ug/ml) for 1, 2, or 5 minutes. 30 to 120 Joules/an of collimated 514.5 nm Argon laser light re delivered to the locier concentrations of 10, 50, and 100 ug,'ml DHE treated cells. One hundre1 fifteen explants were treated, in all. Higher concentrations of DHE alone (500 and 1000 ug/ml) were sufficient to induce cellular swelling. Lower concentrations required light for cellular effect. Trypan blue staining revealed cell death at these minimal pa9ieters: DHE 50 ug/ml, incubation 1 minute, 514.5 r Argon light 1.0 Watt/an for 30 sec (30 Joules) . In future studies, these rameters will be tested in vivo, for their ability to eliminate lens epithelial proliferation after cataract surgery.

Citation Download Citation

Robert W. Lingua M.D., Jean-Marie A. Parel, Gabriel Simon, and Kam Li "Photodynamic treatment of lens epithelial cells for cataract surgery", Proc. SPIE 1423, Ophthalmic Technologies, (1 June 1991); https://doi.org/10.1117/12.43962

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