Nano-sieve-based on-chip particle concentration for nucleic acid sensing

Xinye Chen; Li Liu; Jorge Lomeli-Prieto; Jessica Gu; Jefferson Li; Sharon Ma; Ke Du

doi:10.1117/12.3001181

12 March 2024 Nano-sieve-based on-chip particle concentration for nucleic acid sensing

Xinye Chen, Li Liu, Jorge Lomeli-Prieto, Jessica Gu, Jefferson Li, Sharon Ma, Ke Du

Proceedings Volume 12837, Microfluidics, BioMEMS, and Medical Microsystems XXII; 128370A (2024) https://doi.org/10.1117/12.3001181
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

A miniatured, portable, cost-effective but powerful nano-sieve device is produced and developed for concentrating the targets at low concentrations, leading to the disease diagnostics at point-of-care (POC) settings. To achieve the efficient on-chip concentration of nanoparticles, the multi-sized micro-beads stacking is introduced to this nano-sieve system. Particularly, a flexible PDMS cap is applied to trap the micro-beads in the channel to serve as a three-dimensional filter to physically block and concentrate those nanoparticles, meanwhile allowing the buffer solution to flow through the channel. By coupling with a CRISPR-based assay, our nano-sieve device was expected to be employed for molecular biosensing applications, such as the detection of low light nanoparticles and target RNA, aiming to provide a rapid and efficient multiplexing sample processing at POC environments.

Conference Presentation

Citation Download Citation

Xinye Chen, Li Liu, Jorge Lomeli-Prieto, Jessica Gu, Jefferson Li, Sharon Ma, and Ke Du "Nano-sieve-based on-chip particle concentration for nucleic acid sensing", Proc. SPIE 12837, Microfluidics, BioMEMS, and Medical Microsystems XXII, 128370A (12 March 2024); https://doi.org/10.1117/12.3001181

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