Smart procedures for the femtosecond laser-based fabrication of polymeric lab-on-a-chip

Annalisa Volpe; Maria Serena Chiriacò; Petra Paiè; Filippo Maria Conte Capodacqua; Caterina Gaudiuso; Elisabetta Primiceri; Francesco Ferrara; Roberto Osellame; Antonio Ancona

doi:10.1117/12.2648217

17 March 2023 Smart procedures for the femtosecond laser-based fabrication of polymeric lab-on-a-chip

Annalisa Volpe, Maria Serena Chiriacò, Petra Paiè, Filippo Maria Conte Capodacqua, Caterina Gaudiuso, Elisabetta Primiceri, Francesco Ferrara, Roberto Osellame, Antonio Ancona

Author Affiliations +

Proceedings Volume 12409, Laser-based Micro- and Nanoprocessing XVII; 1240916 (2023) https://doi.org/10.1117/12.2648217
Event: SPIE LASE, 2023, San Francisco, California, United States

Abstract

Development of new lab-on-a-chip (LoC) devices requires an optimization phase in which it could be necessary to continuously modify the architecture and geometry. However, this is only possible if easy, controllable fabrication methods and low-cost materials are available. For this reason, rapid prototyping approaches for the fabrication of polymeric LoC are on the rise, as they allow high degrees of precision and flexibility. Here, we describe the fabrication platform of polymeric microfluidic devices, from the design (CAD) to the proof-ofconcept application as LoC for biological applications. The fabrication procedure is mainly based on fs-laser micromachining techniques. The ability of femtosecond (fs)-laser pulses to produce localized modification of the materials, thereby avoiding either debris, recast layers or unsought thermal affected zones, without restriction of the substrate materials, makes this technology particularly suitable for microfluidic device fabrication. In our work, fs-laser has been also possibly combined with other techniques, without the need for the expensive masks and facilities required by the lithographic process. The LoC devices have been realized in polymethyl methacrylate (PMMA), a low cost and biocompatible material. The fs-based smart fabrication platform has been exploited in the fabrication of disposable LoC devices for particles manipulation. In particular, a serpentine microchannel able to distinguish cancer from non-cancer cells without labeling and a fully inertial sorting 3D device have been fabricated and tested.

Conference Presentation

Citation Download Citation

Annalisa Volpe, Maria Serena Chiriacò, Petra Paiè, Filippo Maria Conte Capodacqua, Caterina Gaudiuso, Elisabetta Primiceri, Francesco Ferrara, Roberto Osellame, and Antonio Ancona "Smart procedures for the femtosecond laser-based fabrication of polymeric lab-on-a-chip", Proc. SPIE 12409, Laser-based Micro- and Nanoprocessing XVII, 1240916 (17 March 2023); https://doi.org/10.1117/12.2648217

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