Single-shot photoluminescence lifetime imaging for fast wide-field optical thermometry

Xianglei Liu; Artiom Skripka; Yingming Lai; Cheng Jiang; Jingdan Liu; Fiorenzo Vetrone; Jinyang Liang

doi:10.1117/12.2649545

16 March 2023 Single-shot photoluminescence lifetime imaging for fast wide-field optical thermometry

Xianglei Liu, Artiom Skripka, Yingming Lai, Cheng Jiang, Jingdan Liu, Fiorenzo Vetrone, Jinyang Liang

Proceedings Volume 12390, High-Speed Biomedical Imaging and Spectroscopy VIII; 123900C (2023) https://doi.org/10.1117/12.2649545
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Photoluminescence lifetime imaging of upconverting nanoparticles is increasingly featured in recent progress in optical thermometry. Despite remarkable advances in photoluminescent temperature indicators, existing optical instruments lack the ability of wide-field photoluminescence lifetime imaging in real time, thus falling short in dynamic temperature mapping. Here, we have developed single-shot photoluminescence lifetime imaging thermometry (SPLIT), which is developed from a compressed-sensing ultrahigh-speed imaging paradigm. Using the core/shell NaGdF₄:Er³⁺,Yb³⁺/NaGdF₄ upconverting nanoparticles as the lifetime-based temperature indicators, we apply SPLIT in longitudinal wide-field temperature monitoring beneath a thin scattering medium. SPLIT also enables video-rate temperature mapping of a moving biological sample at single-cell resolution.

Conference Presentation

Citation Download Citation

Xianglei Liu, Artiom Skripka, Yingming Lai, Cheng Jiang, Jingdan Liu, Fiorenzo Vetrone, and Jinyang Liang "Single-shot photoluminescence lifetime imaging for fast wide-field optical thermometry", Proc. SPIE 12390, High-Speed Biomedical Imaging and Spectroscopy VIII, 123900C (16 March 2023); https://doi.org/10.1117/12.2649545

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available