Quantitative molecular absorption of cosmetic active molecules in human skin using coherent Raman imaging (CARS and SRS)

Barbara Sarri; Xueqin Chen; Anne Potter; Sebastien Gregoire; Thomas Bornschlögl; Hervé Rigneault

doi:10.1117/12.2648080

17 March 2023 Quantitative molecular absorption of cosmetic active molecules in human skin using coherent Raman imaging (CARS and SRS)

Barbara Sarri, Xueqin Chen, Anne Potter, Sebastien Gregoire, Thomas Bornschlögl, Hervé Rigneault

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Proceedings Volume 12357, Visualizing and Quantifying Drug Distribution in Tissue VII; 1235708 (2023) https://doi.org/10.1117/12.2648080
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

We introduce and validate a framework for imaging and quantifying active molecule penetration into human skin ex vivo and in vivo. Our approach combines nonlinear imaging microscopy modalities, such as two-photon excited auto-fluorescence (TPEF) and coherent anti-Stokes Raman scattering (CARS) and stimulated Raman scattering (SRS). The imaging framework is exemplified first on topically applied glycerol diluted in various vehicles such as water and xanthan gel and second, on retinol applied on ex vivo excised human skin. The proposed framework allows for the 3D percutaneous monitoring of active cosmetic molecules and their formulation across human skin. We demonstrate the reservoir property of topically applied xanthan gel vehicle that releases glycerol continuously over time together with the hydrophobic penetration of retinol between corneocyte.

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Barbara Sarri, Xueqin Chen, Anne Potter, Sebastien Gregoire, Thomas Bornschlögl, and Hervé Rigneault "Quantitative molecular absorption of cosmetic active molecules in human skin using coherent Raman imaging (CARS and SRS)", Proc. SPIE 12357, Visualizing and Quantifying Drug Distribution in Tissue VII, 1235708 (17 March 2023); https://doi.org/10.1117/12.2648080

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KEYWORDS

Coherence imaging

Skin

Molecules

CARS tomography

Absorption

Raman spectroscopy

Auto-fluorescence imaging

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