A virus can cause various serious diseases, some of which could destroy entire countries geographically and economically by sweeping infections that were resistant to established treatment. In an attempt to fight a virus, the immune system produces antibodies (IgM/IgG proteins), and the presence of these antibodies is linked to the presence of the infection in the patient. Detecting and measuring the presence of these antibodies would be incredibly valuable for population control and surveillance. However, developing an automated microfluidic immunoassay testing device typically requires a complicated and time-consuming robotic fluid handling technique, which is both expensive and impractical for point-of-care testing. In this study, we demonstrate an integrated microfluidic system, where sample mixing, loading, and detection can be conducted automatically by a smartphone device. Furthermore, we design a 3D printed sample cartridge module, where only 25 μL of a blood sample can be collected to perform the antibody detection test. Additionally, as a feasibility study, we demonstrate sample mixing and detection performance of our device, where the sample mixing is conducted by bidirectional rotation, and the detection efficiency is evaluated by confining cells within a focal plane by rotating the sample cartridge at high speed.
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