A microfabricated microfluidic bioMEMS device to model human brain aneurisms:  the aneurysm-on-a-chip

Lisa M. Reece; Jian Wei Khor; Raviraj Thakur; Ahmed Amin; Steven T. Wereley; James F. Leary

doi:10.1117/12.2076037

5 March 2015 A microfabricated microfluidic bioMEMS device to model human brain aneurisms: the aneurysm-on-a-chip

Lisa M. Reece, Jian Wei Khor, Raviraj Thakur, Ahmed Amin, Steven T. Wereley, James F. Leary

Proceedings Volume 9320, Microfluidics, BioMEMS, and Medical Microsystems XIII; 93200P (2015) https://doi.org/10.1117/12.2076037
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Aneurysms are pockets of blood that collect outside blood vessel walls forming dilatations and leaving arterial walls very prone to rupture. There is little information concerning the causes of intracranial aneurysm formation, growth, and rupture. Current treatments include: (1) clipping, and (2) coil embolization, including stent-assisted coiling. Further, the evolution of any aneurysm is assumed to be caused by the remodeling of the affected blood vessel’s material constituents (tunica intima, tunica media, or tunica adventitia). Velocity, pressure, and wall shear stresses aid in the disease development of aneurysmal growth, while the shear force mechanisms effecting wound closure are elusive. To study aneurysm pathogenesis, a lab-on-a-chip device is the key to discovering the underlying mechanisms of these lesions. A two-dimensional microfluidic model, the Aneurysm-on-a-Chip™ (AOC), was the logical answer to study particle flow within an aneurysm “sac”. The AOC apparatus can track particles/cells when it is coupled to particle image velocimetry software (PIV) package. The AOC fluid flow was visualized using standard microscopy techniques with commercial microparticles and human aortic smooth muscle cells (HASMC). Images were taken during fluid flow experiments and PIV was utilized to monitor the flow of particles within the “sac” region, as well as particles entering and exiting the device. Quiver plots were generated from fluid flow experiments using standard 7 μm latex particles and fixed HASMC in PBS. PIV analysis shows that the particles flowed nicely from input to output. Wall shear stress provided evidence that there was some back flow at the edges of the “sac” – an indicator of aneurysm development in human patients.

Citation Download Citation

Lisa M. Reece, Jian Wei Khor, Raviraj Thakur, Ahmed Amin, Steven T. Wereley, and James F. Leary "A microfabricated microfluidic bioMEMS device to model human brain aneurisms: the aneurysm-on-a-chip", Proc. SPIE 9320, Microfluidics, BioMEMS, and Medical Microsystems XIII, 93200P (5 March 2015); https://doi.org/10.1117/12.2076037

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