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2 March 2022 Fluorescence-detected mid-infrared photothermal microscopy
Aleksandr Razumtcev, Minghe Li, Ruochen Yang, Lynne S. Taylor, Garth J. Simpson
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Proceedings Volume PC11973, Advanced Chemical Microscopy for Life Science and Translational Medicine 2022; PC119730G (2022) https://doi.org/10.1117/12.2609722
Event: SPIE BiOS, 2022, San Francisco, California, United States
Abstract
Fluorescence-detected photothermal mid-infrared (F-PTIR) microscopy is demonstrated experimentally and applied to characterize the chemical composition within micrometer-size phase-separated domains of ritonavir/copovidone amorphous solid dispersions formed upon water sorption. In F-PTIR, temperature-sensitive changes in fluorescence quantum efficiency report on highly localized absorption of mid-infrared radiation. Two-photon excited ultraviolet autofluorescence supported label-free F-PTIR microscopy of tryptophan microcrystals and lyophilized lysozyme particles. F-PTIR provides two degrees of chemical specificity, informing on infrared absorption selectively in the local environments immediately adjacent to fluorescent regions of interest.
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Aleksandr Razumtcev, Minghe Li, Ruochen Yang, Lynne S. Taylor, and Garth J. Simpson "Fluorescence-detected mid-infrared photothermal microscopy", Proc. SPIE PC11973, Advanced Chemical Microscopy for Life Science and Translational Medicine 2022, PC119730G (2 March 2022); https://doi.org/10.1117/12.2609722
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KEYWORDS
Microscopy
Mid-IR
Absorption
Infrared spectroscopy
Luminescence
Particles
Product engineering
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