Label-free histological imaging of skin cancers with photoacoustic remote sensing virtual histology

Benjamin R. Ecclestone; Kevan Bell; Marian Boktor; Vladimir Pekar; Muba Taher; Deepak Dinakaran; John R. Mackey; Parsin Haji Reza

doi:10.1117/12.2609176

7 March 2022 Label-free histological imaging of skin cancers with photoacoustic remote sensing virtual histology

Benjamin R. Ecclestone, Kevan Bell, Marian Boktor, Vladimir Pekar, Muba Taher, Deepak Dinakaran, John R. Mackey, Parsin Haji Reza

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Proceedings Volume PC11934, Photonics in Dermatology and Plastic Surgery 2022; PC119340F (2022) https://doi.org/10.1117/12.2609176
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

A Photoacoustic Remote Sensing (PARS) microscopy system is presented for label-free histological imaging of skin tissues. PARS captures label-free optical absorption contrast visualizing cell nuclei, connective tissues, lipids, and other structures with contrast analogous to common histochemical stains. Imaging is demonstrated in unprocessed human skin with basal cell carcinoma, squamous cell carcinoma, and melanoma. Visualizations are compared against traditional tissue preparation with histochemical staining. Preliminary clinical studies show comparable diagnostic quality between PARS histology and standard histology preparations. The proposed method is a milestone in this translational research project, aiming towards clinical deployment of a PARS microscope for skin cancer imaging.

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Benjamin R. Ecclestone, Kevan Bell, Marian Boktor, Vladimir Pekar, Muba Taher, Deepak Dinakaran, John R. Mackey, and Parsin Haji Reza "Label-free histological imaging of skin cancers with photoacoustic remote sensing virtual histology", Proc. SPIE PC11934, Photonics in Dermatology and Plastic Surgery 2022, PC119340F (7 March 2022); https://doi.org/10.1117/12.2609176

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KEYWORDS

Skin cancer

Photoacoustic spectroscopy

Remote sensing

Tissues

Surgery

Skin

Tumors

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