The molecular characterization of ABO blood types, which is clinically significant in blood transfusion, has clinical and
anthropological importance. Polymerase chain reaction sequence-based typing (PCR-SBT) is one of the most commonly
used methods for the analysis of genetic bases of ABO blood types. However, such methods as PCR-SBT are
time-consuming and are high in demand of equipments and manipulative skill. Here we showed that membrane
electrophoresis based SERS method employed for studying the molecular bases of ABO blood types can provide rapidand
easy-operation with high sensitivity and specificity. The plasma proteins were firstly purified by membrane
electrophoresis and then mixed with silver nanoparticles to perform SERS detection. We use this method to classify
different blood types, including blood type A (n=13), blood type B (n=9) and blood type O (n=10). Combination of
principal component analysis (PCA) and liner discriminant analysis (LDA) was then performed on the SERS spectra of
purified albumin, showing good classification results among different blood types. Our experimental outcomes represent
a critical step towards the rapid, convenient and accurate identification of ABO blood types.
Due to its high sensitivity, flexibility, and “fingerprints” sensing capability, Surface-enhanced Raman Spectroscopy
(SERS) is a very powerful method for characterization of substances. In this paper, two kinds of Radix Astragali with
different quality were firstly extracted through continuous circumfluence extraction method and then mixed with silver
nanoparticles for SERS detection. Most Raman bands obtained in Radix Astragali extraction solution are found at
300-7000cm-1 and 900-1390 cm-1. Although, major peak positions at 470, 556, 949, 1178 and 1286 cm-1 found in these
two kinds of Radix Astragali appear nearly the same, Raman bands of 556 and 1178 cm-1 are different in intensity, thus
may be used as a characteristic marker of Radix Astragali quality. In detail, we can make full use of the different
intensity of two different kinds but the same state at 556 cm-1 to describe the quality standard of Radix Astragali. Our
preliminary results show that SERS combining with continuous circumfluence extraction method may provide a direct,
accurate and rapid detection method of Radix Astragali.
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