With the continuous impact of COVID-19, the demand for rapid genetic diagnosing at the inspection and quarantine site, emergency treatment of sudden infectious diseases and clinical in vitro diagnosis was increasing rapidly. In order to achieve rapid, automatic nucleic acid extraction and detection, an automatic diagnostic system which integrates nucleic acid extraction, amplification and biochip fluorescence detection is designed. The diagnostic system designed based on multidisciplinary intersection of biology, optoelectronics, machinery and computer technology. At first, the nucleic acid extraction part has 1-8 sample flux and uses the principle of the silicon filter can adsorb nucleic acid at different pH to achieve nucleic acid extraction. After the extraction process, the nucleic acid is injected into biochip through robotic arm. There is a specific microarray reaction chamber on the chip, which can be combined with a specific biological substance; Secondly the amplification part used the principle of flat-plate PCR to achieve nucleic acid amplification in the biochip; After amplification, the nucleic acid detection realized under the irradiation of the excitation light at 530nm, the specificity target on the biochip will emit light, thus the fluorescence image scanned by COMS camera and the result analyzed by the software on PC; At last device control, automation and detection of the integrated device realized by the STM32 single-chip microcomputer and CAN communication method to establish a device communication network. The diagnostic system will automate workflow, and provides a result that reports on the detection and interpretation of targets in the samples. The imaging module resolution was less than 10 microns/pixel. The CV value of the sample was less than 10%. The results provide that the diagnostic system can provide more accurate and more automated equipment for nucleic acid extraction, amplification and detection.
For the new satellite autonomous navigation technology using sensors with functions of a star sensor and an ultraviolet earth sensor, it lacks the device to simulate target characteristics of stars and the earth. In the paper, we proposed a combined simulation device composed with a visible light star simulator and an ultraviolet light earth simulator. At first, the general optical system designing program of simulators was presented after analysis of working requirements. Then, the designing process of optical systems was described in detail. After that, high precision and profile simulation method was analyzed intensively. Furthermore, an accurate correction method for the angular travel error between two stars and earth flare angle error was lay out. At last, the visible light stars and ultraviolet light earth combined simulation device was utilized. The results show that the precision of combined simulation device which can simulate star positions and earth graphics is high. Moreover, the angular travel error between two stars is less than ±7 second of arc. In addition, earth flare angle error is less than ±0.05°. The results meet the function and precision requirements for autonomous navigation of an attitude sensor on satellite. The high precision combined simulation device may meet the basic requirements for ground precision calibration and functional test of an attitude sensor.
In clinical testing, with the increasing demand for quantitative analysis of urine protein in departments such as urology, ICU (intensive care unit), a fast, accurate and simple method for detecting early renal impairment attract more and more scholars’ and businesses’ wide attention. This study proposes an early renal function damage detection device that combines ACR analysis and NGAL (neutrophil gelatinase-associated lipocalin) analysis, which could optimizes existing detection methods[1], and improves existing detection techniques. This study based on Lambert-Beer's law, used immuno-transmission colorimetry and immunoturbidimetric turbidity to analyze the samples and build experimental tooling.
Gene biochip imaging and scanning technology is a more advanced gene diagnosis technology after fluorescence quantitative PCR technology, second-generation sequencing technology and digital PCR technology. Due to the great influence of fluorescence microscopy system, the imaging and scanning technology of gene biochip with high imaging efficiency and accuracy has become a hot topic widely concerned by scholars. In this paper, two-channel laser was used as the excitation light source of the biochip, and the fluorescence imaging system of the switchable fluorescence filter was designed to realize the detection of FAM and HEX biological fluorescence signals; According to the working principle of the gene biochip imager, the finite element analysis method is used to analyze the thermal structure of the optical system, and the internal structural parameters of the optical system are optimized according to the simulation results. According to the optimization result, the illumination time of each object pixel is controlled to utilize the fluorescence information more efficiently, and the fluorescent bleaching effect is reduced without sacrificing the image quality, and the PCR amplification oil droplet quantitative reading and display is performed in real time. The gene biochip imaging system proposed in this paper can be used to image, obtain, read, process and display the detected chip with a resolution of 10 m/ pixel, a minimum detection limit of < 10 fluorescent molecules/square micron, and a sample detection repeatability of < 10%.The results show that the gene biological chip imaging system designed by this research has the advantages of low noise, low cost and fast scanning speed, and provides a theoretical basis for precision medical fields such as early diagnosis and cure evaluation of clinical tumors. The results show that the gene biochip imaging system designed by this research has the advantages of low noise, low cost and fast scanning speed, and provides a theoretical basis for precision medical fields such as early diagnosis and cure evaluation of clinical tumors.
In the high-energy laser test system, surface profile and finish of the optical element are put forward higher request. Taking a focusing aspherical zerodur lens with a diameter of 100mm as example, using CNC and classical machining method of combining surface profile and surface quality of the lens were investigated. Taking profilometer and high power microscope measurement results as a guide, by testing and simulation analysis, process parameters were improved constantly in the process of manufacturing. Mid and high frequency error were trimmed and improved so that the surface form gradually converged to the required accuracy. The experimental results show that the final accuracy of the surface is less than 0.5μm and the surface finish is □, which fulfils the accuracy requirement of aspherical focusing lens in optical system.
With the development of advanced optical processing technology, the off-axis aspherical lens are the vital optical device for the high precision measurement and machining system. Off-axis aspherical lens can not only reduce the complexity of the system, but also increase the breadth of coverage to get closer to the ideal image. In order to get high efficiency and high surface accuracy, the off-axis aspherical lens processing and testing methods are deeply researched. The processing of traditional off-axis aspherical lens process coaxial aspherical lens first, then cut it to off-axis aspherical lens. This method is prone to edge spliting and on the surface of the secondary damage. This article presents a novel processing technology - splicing processing technology. The technology can guarantee the accuracy of polishing the surface type, and to avoid secondary damage to the surface, to achieve high-precision off-axis aspherical lens machining. The off-axis aspherical lens are tested by Taylor Hobson profilometer. The off-axis aspheric surface figure can get 0.715 μm, the surface polish can achieve the Grade II. Therefore, a high efficiency, reproducible off-axis aspherical polishing method were summarized. The off-axis aspherical lens to achieve mass production.
Aspherical cylindrical lens compared with the cylindrical lens, they improved image quality and optical properties, simplified the system architecture. They applied in many fields, such as high power laser system, fax machines and typographical scan imaging system, as well as bar code scanning, lighting and other aspects of holography. Aspherical cylindrical lens are centrosymmetric. It is difficult to process. Parallel with the side line and bus bar line is difficult to ensure. Machining accuracy is low. It is usually about 15 μm, that not sufficient to meet the needs of modern highprecision laser systems. These have become a major problem restricting its development. Combining traditional and modern polishing techniques, a new technique for polishing aspherical cylindrical lens is proposed-- longitudinal feedback compensation technology. With dimensions of 15 × 5 × 5 mm quartz aspherical cylindrical lens as an example, the surface profilometer results of detection of the workpiece usually, modify the shape of the polishing surface of the mold, to control the size of the area of the polishing, the surface of the workpiece to achieve the effect of the type of compensation. After repeated testing and feedback compensation, gradually improve the accuracy of the workpiece surface type. The results show that this technique can effectively improve the precision aspherical cylindrical lens. After detection the workpiece surface accuracy is 0.8μm, the surface finish is Class II. It has the actual production of a certain application value.
In recent years, with the development of space technology, the infrared earth simulator that can only provide an earth angle could not meet the requirements of the calibration tests for earth sensor on the ground, then the research on infrared earth simulator which can provide a variety of earth angles has become an inevitable trend. Based on the collimated infrared earth simulator’s working principle and design requirements, aimed at these three orbit height 18000Km, 35786 Km and 42000 Km, a high altitude orbits collimating infrared earth simulator is developed by adopting the collimation and the earth diaphragm alterable project. Applied ZEMAX software to design a meniscus of germanium collimating lens, the simulation results show that the lens has a effective aperture of 240mm, provide the optimum position and diameter of the earth diaphragms correspond following three earth angles of 30.42°, 17.46° and 15.19°. Used ANSYS software to do finite element analysis for the key components of the mechanical structure. Finally, validate the deviation accuracy of flare angles and beam parallelism via theoretical analysis and practical calculation. The results indicate that the errors of three flare angles of the earth are all less than ±0.05, can meet the accuracy requirements of infrared earth simulator for infrared earth sensor’s calibration testing on the ground. Key words: Infrared earth sensor, Earth simulator, Variable angular, Germanium collimating lens
During the runtime of the spacecraft in space, spacecraft uses optical navigation sensor to capture and measure its flight attitude information.Very high accuracy star sensor is a kind of navigation sensors.Star simulator serves as the calibration equipment for star sensor on the ground has received more and more attention.In order to complete the testing of very high star sensor, according to the working principlestatic of the star simulator,an collimating optical system has been designed which can achieve high precision simulation of the star point.Using ZEMAX software to complete the design of the optical system and calculating the star point emitting precision at -45 °C ~ +65 °C with multiple structural function.The design results show that,the designed wavelength of the system is 500~900nm,wherein the center wavelength is 680nm.At 20°C,the distortion is 0.01%, MTF is higher than 0.5 at the frequency of 60 lp/mm within the filed of view of 7.2°.The collimation errors are both less than 1″ at the two limit temperatures.Applying ANSYS software to analysis the deformation of machine structure at different temperatures, the result shows that the overall deformation has the same tendency as the lens group,and can meet temperature requirements. Then making the precision analysis of the optical system and providing a group of measured data. Depended on the measurement results, the error of position for single star ≤7″, the error of angular distance between stars≤10″. The data shows that this design and the product meet the requirement of technique index.
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