In this study, a detection system based on Polarization-Sensitive Optical Coherence Tomography (PS-OCT) using Mueller Matrix Optical Coherence Tomography (MM-OCT) was developed. By employing PS-OCT technology, the system was able to fully detect all sixteen elements of the Mueller matrix. By comparing the intensity element M00 among the sixteen elements of the Mueller matrix, the texture structure of the pearl layers could be observed. This allowed for differentiation between freshwater and saltwater pearls, identification of genuine and fake pearls, detection of internal flaws in pearls, and differentiation between nucleated and non-nucleated pearls. The study also involved the labeling of connected regions in binary images, where pixels within the same connected region were assigned the same label. The labeled images were displayed to facilitate more intuitive qualitative analysis, and quantitative analysis was performed using gray-level co-occurrence matrices. Subsequently, pearl layer pixels were extracted from multiple angles in the images, and the thickness of the pearl layer was calculated using the extracted pixels and axial resolution. Finally, detection and classification of unknown pearls were conducted, yielding results consistent with the actual outcomes. The measured thickness results after sectioning matched the calculated results, providing evidence for the feasibility of the experimental method proposed in this study.
Malignant tumor is a serious threat to human health. With the development of medical technology, a variety of treatment methods appear in clinic. As a non-invasive treatment, laser photothermal therapy is a treatment that kills cancer cells by converting light energy into heat energy through laser irradiation. Its advantage is protecting normal tissue while destroying cancerous tissue. However, it’s still not clear that the effect of heat generated by laser on tissue and temperature changes during photothermal treatment process. Optical coherence tomography (OCT) is a non-contract, real-time optical imaging technology. OCT has been widely used in clinical treatment and scientific research based on fast imaging speed and high detection sensitivity. In our study, breast cancer of mice was chosen as the research object. Combined infrared thermography and OCT were applied to monitor the dynamic changes of tumor tissue. The effect of photothermal from OCT image and temperature were obtained and analyzed. Specifically, we investigated the structural change characteristics and temperature distribution of tumor tissue with increasing laser power. And then, the temperature change of tumors of different sizes at power of 3W were further analyzed. The results show that combined with OCT images and temperature can be well used to guide the photothermal treatment process. It can serve as a basis for the method with safely, consistently and effectively.
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