KEYWORDS: Optical coherence tomography, 3D image processing, 3D metrology, In vivo imaging, Cameras, Retinal scanning, Retina, Spectroscopy, 3D acquisition, Motion measurement
Line-field spectral domain optical coherence tomography (LF-SDOCT), which is a parallel detection OCT technique
that provides single cross-sectional image without mechanical scanning, has been developed for very high-speed three-dimensional
(3D) imaging of human retina in vivo. By optimizing the integration time of the camera for the sample
motion, we have successfully performed the in vivo 3D retinal imaging, which is sufficiently free from the motion artifact.
The achievable A-lines rate is 54.4 kHz A-lines/s with the maximum sensitivity of 89.4 dB, and it corresponds to more
than 2 times higher speed than conventional flying spot SD-OCT system. The in vivo 3D retinal measurement with the 256
cross-sectional images (528 A-lines/image) was successfully performed at 2.5 seconds.
We present three dimensional (3D) imaging of macular diseases and glaucoma with high speed, Fourier domain optical coherence tomography (FD-OCT). Our FD-OCT system allows video rate cross-sectional imaging with 98 dB sensitivity and 4.3 μm depth-resolution in tissue. This performance results in high contrast sectional images that enhance visualization of fine retinal layers including external limiting membrane and of deep structure such as the choroid and optic nerve. Volume rendering of 3D OCT data set taken for 3.5 seconds provides realistic 3D images of macular, optic disc and their pathologic changes. This manuscript will show the methods for three dimensional FD-OCT including a raster scanning protocol for volume rendering and cancellation of the motion artifact of eye balls, and the application of the high contrast three dimensional OCT imaging to macular diseases and glaucoma in clinical examination.
KEYWORDS: Optical coherence tomography, Skin, Image segmentation, In vivo imaging, Tomography, Image processing algorithms and systems, Detection and tracking algorithms, Light sources, Signal detection, Binary data
After segmentation of the epidermis from three-dimensional coherence tomography volume, a depth-oriented algorithm provides a segmentation of the infundibulum. In this process, the epidermal thickness, the population and the occupation ratio of the infundibula are provided.
KEYWORDS: Optical coherence tomography, Cameras, In vivo imaging, 3D acquisition, Spectroscopy, 3D image processing, Tissues, Camera shutters, Tomography, Visualization
A high-speed line-field Fourier-domain optical coherence tomography system has been developed. Tomographic images consisting of 656 A-lines are obtained at 121 frames per second. It is corresponding to 79,400 A-line/s. Three-dimensional volume sets consist of 256 OCT images are measured within 2.1 seconds. The sensitivity of this system is 79.5 dB. A biological tissue measurement is demonstrated with human nail fold in vivo. The three-dimensional nail fold structure is visualized.
Polarization sensitive Fourier domain optical coherence tomography (PS-FD-OCT) using fiber components with continuous polarization modulation is demonstrated.
The incident polarized light is modulated by electro-optic modulator (EO modulator) synchronized with lateral B-scanning.
By the incident polarization modulation and the polarization sensitive spectrometer, the depth-resolved Jones matrix image of biological sample can be measured.
This method uses both polarization modulation method and Fourier transform method.
In this paper, the algorithm is described and the phase retardation image of chicken breast muscle is measured.
A numerical deconvolution method that cancels the blurring due to lateral defocus in line field Fourier domain optical coherence tomography (LF-FDOCT) using imaging optics is proposed. This method employs an inverse filter designed from the point spread function (PSF) that is calculated by Fresnel diffraction. The inverse filter can eliminate the lateral defocus, and consequently, the out-of-focus lateral resolution can be improved to a level comparable with the in-focus resolution over the entire axial measurement range. In this paper, we describe the process of calculating the PSF and the inverse filter designed from it in LF-FDOCT. The effect of deconvolution is also schematically discussed and estimated. A knife-edge method also verifies the effect of in-focus resolution experimentally.
KEYWORDS: Optical coherence tomography, Deconvolution, Image segmentation, Point spread functions, Resolution enhancement technologies, Super resolution, Signal detection, In vivo imaging, Eye, Infrared imaging
A method of lateral superresolution for Fourier domain optical coherence tomography is presented. This method consists of intentional defocus and its numerical compensation using a spatial frequency- phase filter. The designing process of the phase filter is described, and the superresolution effect is discussed theoretically. Experimental results of a knife-edge test prove that the frequency filter enhances the lateral resolution better than a diffraction-limited resolution. This method is applied to the investigation of an in vivo human iris and shows the effect of the cancellation of defocus.
Standard high-speed Fourier domain optical coherence tomography
(FD-OCT) and a modified version of FD-OCT; a line-field FD-OCT
(LF-FDOCT) are demonstrated.
LF-FDOCT is using the principle of FD-OCT for its depth resolution and
a one-dimensional imaging optics for its one-dimensional lateral
resolution.
A mechanical C-scan drived by a galvano scanner is introduced into the
FD-FDOCT, which shows a cross sectional OCT image without any
mechanical scanning.
The improved version of FD-OCT visualizes the three-dimensional
structure of a sample with only one-dimensional scanning.
Both standard FD-OCT and LF-FDOCT are applied to dermatological
applications and visualize the inner structure of an in vivo
human fingerpad.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.