The hyperspectral reflectance of canopy of winter wheat and data of leaf nitrogen accumulation (LNA) were acquired in
primary growth stages under different nitrogen levels in order to monitor winter wheat status and diagnose nitrogen
using remote sensing method. A new method was developed to estimate the nitrogen nutrition of winter wheat using
continuum-removed method, which generally used in spectra analysis on rock and mineral. The continuum-removed
method was effectively used to magnify the object spectral absorption features, and it could be convenient to extract the
spectral absorption features. Based on the continuum-removed treatment and the correlation between absorption feature
parameters and LNA, results show that LNA increased with increasing the nitrogen fertilization. LNA increased from the
erecting stage to the booting stage and decreases from the booting to the heading stage under all nitrogen levels. It is the
VNIR regions that were sensitive to LNA. By continuum removal operation, it can be found that the method magnify the
subtle difference in spectral absorption characteristics arise from the nitrogen stress on winter wheat. At all stages, total
area of absorption peak, left area of absorption peak, right area of absorption peak increased with increasing the nitrogen
fertilization, whereas the normalized maximal absorption depth by area decreased. The correlation analysis indicated that
all the absorption characteristics parameters of continuum-removed spectra highly correlated with LNA, and the
correlation relationship of the whole growth cycle was stronger than that of any single growth stage. But the booting
stage is the best at the several single growth stages and the NMAD is the best absorption parameter to monitoring the
nitrogen of winter wheat canopy. The range 550 nm to 760 nm are the feature bands for extracting nitrogen information
of canopy. The regression analysis on the whole growth period showed that the all regression models between the
absorption characteristics parameters and LNA were all extremely significant (P<0.001).Therefore, continuum-removed
method is a feasible method for quantificational evaluation of winter wheat LNA.
The hyperspectral reflectance for rape fresh leaves and data of chlorophyll and total nitrogen content were acquired in
primary growth stages under different nitrogen levels in order to monitor rape status and diagnose nitrogen using remote
sensing method. A new method was developed for estimating the nitrogen nutrition of rape using continuum-removed
method, which generally used in spectral analysis on rock and mineral. Based on the continuum-removed treatment and
the correlation between absorption feature parameters and total nitrogen content of fresh leaves, results show that
reflectance at the visible region decreased with increasing in the nitrogen fertilization, and continuum-removed
operation can magnify the subtle difference in spectral absorption characteristics arose from the nitrogen stress on rape.
During the seeding stage, bud-emerging stage and flowering stage of rape, total area of absorption peak, area left of the
absorption peak and area right of the absorption peak in 550-750 nm region increased with increasing in the nitrogen
fertilization, but it was opposite for the area-normalized maximal absorption depth. The correlation analysis indicated
that it is at seeding stage that the relation between absorption characteristics parameters and leaf total nitrogen was best
close. The research demonstrated that continuum-removed method is a feasible method for quantificational evaluation
of rape nitrogen nutrition, and the seeding stage of rape is the best stage for assessment of rape nitrogen nutrition based
on absorption characteristics of fresh leaves.
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