A miniature endomicroscope is presented that combines a large field-of-view (up to 1.7 mm) for OCT-imaging and a high-resolution mode with 360 μm field-of-view (NA = 0.5) for multi-photon fluorescence or OCT imaging. The 4.7x magnification variation is achieved by the axial positioning of an inner micro-optical lens group using an integrated electro-magnetic z-actuator. A reverse fiber-optic piezotube-scanner with minimized length is employed for the image acquisition by resonant spiral scanning. With the probe diameter of 2.7 mm and a rigid length of about 60 mm, the approach may pave the way to clinical applications of these two modalities in a single probe.
Light sheet microscopy is a microscopy technique characterized by an illumination from the side, perpendicular to the direction of observation. While this is often used and easy to implement for imaging samples with water-immersion, the application in combination with oil-immersion is less often used and requires a specific optimization. In this paper we present our design of a light-sheet illumination optical system with a ~1μm illumination thickness, a long working distance through the immersion oil, and including a focusing system allowing for moving the focus-spot of the lightsheet laterally through the field of view. This optical design allows for the acquisition of fluorescence images in 3D with isotropic resolution of below 1 micrometer of whole-mount samples with a size of ~1mm diameter. This technique enables high-resolution insights in the 3D structure of biological samples, e.g. for research of insect anatomy or for imaging of biopsies in medical diagnostics.
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