Dr. Uttam K. Sinha Profile

Dr. Uttam K. Sinha

at Keck Medicine of USC

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Author

Publications (4)

Proceedings Article | 13 March 2024 Presentation + Paper

A portable biosensing system using tapered fiber sensors utilizing mode interference

Chaminda Ranathunga, Cong Deng, Partha Banerjee, Casey Collet, Uttam Sinha

Proceedings Volume 12835, 1283507 (2024) https://doi.org/10.1117/12.3005681

KEYWORDS: Refractive index, Beam propagation method, Wave propagation, Cladding, Step index fibers, Fiber optics sensors, Biosensing, Waveguides, Simulations, Sensors

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SPIE Journal Paper | 12 July 2022

Practical tapered optical fiber system for in-situ label-free sensing of various antigens

Cong Deng, Chaminda Ranathunga, Partha Banerjee, Xuelian Chen, Jiang Zhong, Uttam Sinha

OE, Vol. 61, Issue 07, 074102, (July 2022) https://doi.org/10.1117/12.10.1117/1.OE.61.7.074102

KEYWORDS: Tapered optical fibers, Sensing systems, Fiber lasers, Optical engineering, Molecules, Sensors, Refractive index, LabVIEW, Proteins, Signal to noise ratio

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A practical tapered optical fiber (TOF) biosensing system was developed for label-free detection using antigen-antibody pairs with repeatable results and a very high degree of sensitivity. This was done by attaching molecular recognition agents to a tapered fiber surface for augmenting sensitivity and specificity of analyte. The entire system included three main parts: a tunable laser, a tapered fiber, and an optical detector. Light from an unpolarized tunable fiber laser was introduced into the tapered fiber from one end, and the transmitted intensity was detected by a photodetector. In the tapered fiber area, the evanescent electromagnetic field, which extends outside the fiber, was able to detect minute changes in the refractive index caused by antigen-antibody pairs. Recorded data was analyzed using an innovative Fourier analysis method to find phase changes, which are directly related to the biomolecular concentration coated on fiber, from which antibody-antigen concentrations are obtained. Two experiments were performed to confirm the concept using two very different agents. The first was the protein Interleukin-8 (IL-8). Repeatable results with a sensitivity of 10 pg/mL were achieved. The second was human coronavirus OC43 (HCoV-OC43), a surrogate viral particle for SARS-CoV-2, with a sensitivity of 50 viruses/mL. Critical sources of error were identified and addressed for the purpose of using the device for real clinical diagnosis in various real-life environments, where viruses can reside in water, phosphate-buffer solution, or saliva, the most popular three environments in real clinical diagnosis. Our device was designed according to the principle that only one specific kind of antibody and antigen can be combined together. The device demonstrated good accuracy to chosen analyte(s) tailored to specific applications and offered the potential to develop a point-of-care device used in clinics, as well as for detecting a variety of viruses and biocontaminants. The reproducibility of TOFs was confirmed through multiple fabrications and consistent results.

Proceedings Article | 20 February 2020 Paper

Tapered fiber sensor usable for head and neck cancer screening

Cong Deng, Karolyn Hansen, Joseph Haus, Partha Banerjee, Uttam Sinha

Proceedings Volume 11233, 112330Z (2020) https://doi.org/10.1117/12.2545323

KEYWORDS: Fiber optics sensors, Cancer, Molecules, Neck, Optical fibers, Phase shifts, Head, Signal to noise ratio, Refractive index, Biological research

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Proceedings Article | 22 February 2017 Presentation + Paper

Optical trapping of metallic nanoparticles using microbubbles

Ujitha Abeywickrema, Chenglong Zhao, Partha Banerjee, Arjun Krishnappa, Uttam Sinha

Proceedings Volume 10078, 1007808 (2017) https://doi.org/10.1117/12.2255017

KEYWORDS: Nanoparticles, 3D modeling, Silver, Laser development, Laser beam propagation, Gaussian beams, Optical tweezers, Liquids, Charge-coupled devices, Free space optics, Polarizability, Dielectrics, Temperature metrology

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