Mr. Shibajyoti Ghosh Dastider Profile

Shibajyoti Ghosh Dastider

at Univ of Missouri

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Proceedings Article | 5 May 2012 Paper

Impedance biosensor based on interdigitated electrode array for detection of E.coli O157:H7 in food products

Shibajyoti Ghosh Dastider, Syed Barizuddin, Majed Dweik, Mahmoud Almasri

Proceedings Volume 8369, 83690Q (2012) https://doi.org/10.1117/12.920821

KEYWORDS: Biosensors, Electrodes, Bacteria, Capacitance, Gold, Detector arrays, Pathogens, Biological research, Sensors, Microfluidics

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An impedance biosensor was designed, fabricated and tested for detection of viable Escherichia coli O157:H7 in food samples. This device consists of interdigitated microelectrode array (IDEA) fabricated using thin layer of sputtered gold, embedded under a polydimethylsiloxane (PDMS) microchannel. The array of electrodes is designed to detect viable EColi in different food products. The active surface area of the detection array was modified using goat anti-E.coli polyclonal IgG antibody. Contaminated food samples were tested by infusing the supernatant containing bacteria over the IDEA's, through the microchannel. Antibody-antigen binding on the electrodes results in impedance change. Four serial concentrations of E.coli contaminated food samples (3x10² CFUmL^-1 to 3x10⁵ CFUmL^-1) were tested. The biosensor successfully detected the E.coli samples, with the lower detection limit being 3x10³ CFUmL^-1 (up to 3cells/μl). Comparing the test results with an IDEA impedance biosensor without microchannel (published elsewhere) indicates that this biosensor have two order of magnitude times higher sensitivity. The proposed biosensor provides qualitative and quantitative detection, and potentially could be used for detection of other type of bacteria by immobilizing the specific type of antibody.

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