KEYWORDS: Optical sensing, Optical coherence tomography, 3D modeling, Pulmonary disorders, Pathogens, Real time imaging, Lung, In vitro testing, Diagnostics, 3D image processing
Airway organoid is a three-dimensional (3D) epithelial models for human airway where ciliary behaviors are important to indicate their health state. A real-time noninvasive imaging technique to assess the ciliary movement in airway organoids in vitro is highly demanded. Here we propose a new imaging approach to monitor the ciliary beating of airway organoids noninvasively using dynamic contrast optical coherence tomography (DyC-OCT). We employed DyC-OCT to measure the ciliary beating frequency (CBF) and to highlight ciliary beating clusters distribution in 3D. With DyC-OCT, we monitored the morphology change in ciliary beating clusters as well as the change in CBF from the same organoids during the respiratory syncytial virus (RSV) infection. The capability of DyC-OCT in ciliary beating monitoring shows its potential for respiratory disease diagnosis and pathogen assay.
Longitudinal imaging of live organisms is essential to life science research aiming to understand biological processes. Recently, the imaging system has been housed for longitudinal imaging by mounting the optical imaging system in an incubator, while facing the limitations of single-modality and low throughput. Here, we proposed the OCToScope, a multi-modal, high-throughput and compact platform that is compatible with commercial incubators. OCToScope incorporates Optical Coherence Tomography(OCT) to achieve non-invasive three-dimensional imaging, and fluorescent imaging that retrieves cell-specific information. OCToScope also supports automatic scanning of mass samples quantity with motorized x-y-z stage and perform high time-resolution imaging for long-term studies. we used OCToScope to provide a systematic and comprehensive characterization of human heart organoids for over 30 days without any interference.
We developed a three-dimensional motion tracking method based on circular scans with optical coherence tomography. For transverse motion, the detectable speed ranges from millimeters to centimeters per second, while for axial motion, it ranges from micrometers to millimeters per second for axial motion. This method provides fast and high-precision measurements of the sample motion in both magnitude and direction, which will provide active motion compensation via feedback control for in vivo microscopic and macroscopic imaging applications in the future.
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