Optical Coherence tomography (OCT) is a rapidly developing light based imaging modality that can provide functional information and visualization of tissue morphology and cell death by quantifying the motion of intracellular structures. In this work, Dynamic light scattering (DLS) with OCT, is used to compute the time-dependent fluctuations in scattered light intensity. Using the DLS-OCT method, we have previously shown a higher rate of intracellular motion detected in apoptotic cells compared to viable cells. In this study, we aim to probe the intracellular motion of cells at much higher scan-rates that we have attempted previously to detect sub-cellular motion with greater sensitivity. To validate the DLS-OCT approach, M-mode OCT images were acquired using Thorlabs SS-OCT system (sampling rate of 100 kHz) to measure the Brownian motion in monodisperse microsphere suspensions. The results demonstrate that ACF decays more rapidly for smaller size microspheres and the experimental decorrelation time values matched with the theoretical values calculated using the Einstein-Stokes equation.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.