Prof. Sebastian Karpf Profile

Prof. Sebastian Karpf

at Univ of Lübeck

SPIE Involvement:

Author

Publications (23)

Proceedings Article | 9 August 2023 Paper

Paper

Kilohertz two-photon SLIDE microscopy using a newly developed 780nm excitation laser

Tonio Kutscher, Christian Stock, Florian Sommer, Jonas Jurkevicius, Stefan Meyer, Anton Gruber, Annika Hunold, Moritz Wiggert, Christina Leonhardt, Philipp Lamminger, Sebastian Karpf

Proceedings Volume 12630, 126300B (2023) https://doi.org/10.1117/12.2670904

KEYWORDS: Laser development, Crystals, Imaging systems, Signal detection, Pulsed laser operation, Microscopy, Fluorescence lifetime imaging, Diffraction gratings, Biological imaging, Autofluorescence

Read Abstract +

Proceedings Article | 9 August 2023 Paper

Paper

Kilohertz multimodal SLIDE imaging in the visible

C. Stock, T. Kutscher, P. Lamminger, S. Karpf

Proceedings Volume 12630, 126300E (2023) https://doi.org/10.1117/12.2670885

KEYWORDS: Fluorescence, Fluorescence lifetime imaging, Fluorescence imaging, Second harmonic generation, Laser scanners, Image restoration, Multimodal imaging, Laser frequency, Imaging systems, Pulse signals

Read Abstract +

Proceedings Article | 9 August 2023 Paper

Paper

Small footprint SLIDE demonstrator for 40-Hz volume rate multiphoton microscopy

Dirk Theisen-Kunde, Florian Sommer, Veit Danicke, Lion Schützeck, Stefan Meyer, Christopher Kren, Maximilian Rixius, Sebastian Karpf

Proceedings Volume 12630, 126300Q (2023) https://doi.org/10.1117/12.2670881

KEYWORDS: Multiphoton microscopy, Voxels, Microscopes, Biological imaging

Read Abstract +

Proceedings Article | 16 March 2023 Presentation + Paper

Presentation + Paper

Kilohertz two-photon SLIDE microscopy using a newly developed 780 nm excitation laser

Tonio Kutscher, Christian Stock, Florian Sommer, Jonas Jurkevicius, Stefan Meyer, Anton Gruber, Annika Hunold, Moritz Wiggert, Christina Leonhardt, Philipp Lamminger, Sebastian Karpf

Proceedings Volume 12390, 1239007 (2023) https://doi.org/10.1117/12.2650540

KEYWORDS: Crystals, Imaging systems, Pulsed laser operation, Signal detection, Diffraction gratings, Autofluorescence, Two photon excitation microscopy, Pulse signals, Fluorescence lifetime imaging

Read Abstract +

Proceedings Article | 8 March 2023 Presentation + Paper

Presentation + Paper

900 nm swept source FDML laser with kW peak power

Philipp Lamminger, Hubertus Hakert, Simon Lotz, Jan Philip Kolb, Tonio Kutscher, Sebastian Karpf, Robert Huber

Proceedings Volume 12400, 124001I (2023) https://doi.org/10.1117/12.2649663

KEYWORDS: Fiber lasers, Raman spectroscopy, Microscopy, Tunable filters, Photonic crystal fibers, Four wave mixing, Wavelength tuning

Read Abstract +

Proceedings Article | 4 March 2022 Presentation + Paper

Presentation + Paper

Ultra-high-accuracy chromatic dispersion measurement in optical fibers

M. Klufts, S. Lotz, M. Bashir, S. Karpf, R. Huber

Proceedings Volume 11997, 119970L (2022) https://doi.org/10.1117/12.2608773

KEYWORDS: Dispersion, Optical fibers, Modulation, Tunable lasers, Time metrology, Optical testing, Data processing, Femtosecond phenomena

Read Abstract +

Proceedings Article | 5 March 2021 Presentation

Presentation

Time stretch lidar: a fast spectrally scanned time-of-flight 3D camera

Bahram Jalali, Yunshan Jiang, Sebastian Karpf

Proceedings Volume 11684, 116841B (2021) https://doi.org/10.1117/12.2578065

KEYWORDS: Stereoscopic cameras, LIDAR, Video, Line scan image sensors, Control systems, 3D acquisition, Wavelength tuning, Time of flight imaging, Time of flight cameras, Target detection

Read Abstract +

Proceedings Article | 5 March 2021 Presentation

Presentation

Combination of two-photon microscopy and optical coherence tomography with fully fiber-based lasers for future endoscopic setups

Philipp Lamminger, Merle Loop, Julian Klee, Daniel Weng, Jan Philip Kolb, Matthias Strauch, Sebastian Karpf, Robert Huber

Proceedings Volume 11634, 1163405 (2021) https://doi.org/10.1117/12.2578679

KEYWORDS: Optical coherence tomography, Two photon excitation microscopy, Fiber lasers, Endoscopy, Semiconductor lasers, Luminescence, Imaging systems, Fluorescence lifetime imaging, Endoscopes, Cladding

Read Abstract +

Proceedings Article | 5 March 2021 Presentation + Paper

Presentation + Paper

A detailed analysis of the coherence and field properties of an FDML laser by time resolved beat signal measurements

Christin Grill, Torben Blömker, Mark Schmidt, Dominic Kastner, Tom Pfeiffer, Jan Philip Kolb, Wolfgang Draxinger, Sebastian Karpf, Christian Jirauschek, Robert Huber

Proceedings Volume 11665, 116651A (2021) https://doi.org/10.1117/12.2578293

KEYWORDS: Signal detection, Optical coherence tomography, Light sources, Oscilloscopes, Mode locking, Continuous wave operation, Coherence (optics)

Read Abstract +

Proceedings Article | 5 March 2021 Presentation

Presentation

Sectioning-free virtual H&E histology with fiber-based two-photon microscopy

Matthias Strauch, Jan Philip Kolb, Wolfgang Draxinger, Ann-Kathrin Popp, Melanie Wacker, Nadine Merg, Jennifer Hundt, Sebastian Karpf, Robert Huber

Proceedings Volume 11648, 116480V (2021) https://doi.org/10.1117/12.2578334

KEYWORDS: Two photon excitation microscopy, Tissues, Microscopes, Tissue optics, Relays, Pulsed fiber lasers, Photomultipliers, Pathology, Objectives, Mirrors

Read Abstract +

Proceedings Article | 21 February 2020 Presentation + Paper

Presentation + Paper

Beating of two FDML lasers in real time

Christin Grill, Simon Lotz, Torben Blömker, Dominic Kastner, Tom Pfeiffer, Sebastian Karpf, Mark Schmidt, Wolfgang Draxinger, Christian Jirauschek, Robert Huber

Proceedings Volume 11260, 1126014 (2020) https://doi.org/10.1117/12.2545794

KEYWORDS: Optical coherence tomography, Signal detection, Continuous wave operation, Mode locking, Tunable lasers, Tunable filters, Light sources

Read Abstract +

Proceedings Article | 4 March 2019 Presentation

Presentation

Discrete time-stretch LiDAR (Conference Presentation)

Yunshan Jiang, Sebastian Karpf, Bahram Jalali

Proceedings Volume 10908, 109080I (2019) https://doi.org/10.1117/12.2510843

KEYWORDS: LIDAR, Dispersion, Spectrometers, Cameras, Tomography, Microfluidics, Velocimetry, Vibrometry, Target detection, Sensors

Read Abstract +

Proceedings Article | 4 March 2019 Presentation

Presentation

Label-free imaging of tumorous tissue in the Raman fingerprint region with time-encoded (TICO) stimulated Raman scattering (Conference Presentation)

Hubertus Hakert, Matthias Eibl, Sebastian Karpf, Barbara Wollenberg, Ralph Pries, Robert Huber

Proceedings Volume 10882, 108821R (2019) https://doi.org/10.1117/12.2509851

KEYWORDS: Raman spectroscopy, Tissues, Tumors, Raman scattering, Microscopy, Fiber lasers, Signal to noise ratio, Nonlinear optics, Proteins, Biopsy

Read Abstract +

Proceedings Article | 22 February 2019 Paper

Paper

Virtual HE histology by fiber-based picosecond two-photon microscopy

Jan Philip Kolb, Daniel Weng, Hubertus Hakert, Matthias Eibl, Wolfgang Draxinger, Tobias Meyer, Thomas Gottschall, Ralf Brinkmann, Reginald Birngruber, Jürgen Popp, Jens Limpert, Sebastian Nino Karpf, Robert Huber

Proceedings Volume 10882, 108822F (2019) https://doi.org/10.1117/12.2507866

KEYWORDS: Luminescence, Femtosecond phenomena, Picosecond phenomena, Fiber lasers, Optical filters, Tissues, Laser systems engineering, Objectives, Microscopes

Read Abstract +

Proceedings Article | 14 March 2018 Presentation

Presentation

Dynamic chromo-angular-modal excitation of multimode waveguides via acousto-optic deflection (Conference Presentation)

Jacky C. Chan, Sebastian Karpf, Bahram Jalali

Proceedings Volume 10517, 105170C (2018) https://doi.org/10.1117/12.2291418

KEYWORDS: Dispersion, Imaging spectroscopy, Optical components, Waveguides, Acousto-optics, Modulation, Spectroscopy, Waveguide modes, Multimode fibers, Optical calibration

Read Abstract +

Proceedings Article | 28 July 2017 Paper

Paper

Single pulse two-photon fluorescence lifetime imaging (SP-FLIM) with MHz pixel rate and an all fiber based setup

Matthias Eibl, Sebastian Karpf, Hubertus Hakert, Daniel Weng, Tom Pfeiffer, Jan Philip Kolb, Robert Huber

Proceedings Volume 10414, 1041403 (2017) https://doi.org/10.1117/12.2286035

KEYWORDS: Two photon excitation microscopy, FRET and FLIM microscopy, Fiber coupled lasers, Microscopy, Fiber lasers, Fluorescence lifetime imaging, Luminescence, Fiber optics, Nonlinear optics

Read Abstract +

Proceedings Article | 28 July 2017 Paper

Paper

Sparse-sampling with time-encoded (TICO) stimulated Raman scattering for fast image acquisition

Hubertus Hakert, Matthias Eibl, Sebastian Karpf, Robert Huber

Proceedings Volume 10414, 1041408 (2017) https://doi.org/10.1117/12.2287947

KEYWORDS: Raman scattering, Microscopy, Fiber lasers, Image acquisition, Nonlinear optics, Fiber optics, Raman spectroscopy, Mode locking

Read Abstract +

Modern biomedical imaging modalities aim to provide researchers a multimodal contrast for a deeper insight into a specimen under investigation. A very promising technique is stimulated Raman scattering (SRS) microscopy, which can unveil the chemical composition of a sample with a very high specificity. Although the signal intensities are enhanced manifold to achieve a faster acquisition of images if compared to standard Raman microscopy, there is a trade-off between specificity and acquisition speed. Commonly used SRS concepts either probe only very few Raman transitions as the tuning of the applied laser sources is complicated or record whole spectra with a spectrometer based setup. While the first approach is fast, it reduces the specificity and the spectrometer approach records whole spectra -with energy differences where no Raman information is present-, which limits the acquisition speed. Therefore, we present a new approach based on the TICO-Raman concept, which we call sparse-sampling. The TICO-sparse-sampling setup is fully electronically controllable and allows probing of only the characteristic peaks of a Raman spectrum instead of always acquiring a whole spectrum. By reducing the spectral points to the relevant peaks, the acquisition time can be greatly reduced compared to a uniformly, equidistantly sampled Raman spectrum while the specificity and the signal to noise ratio (SNR) are maintained. Furthermore, all laser sources are completely fiber based. The synchronized detection enables a full resolution of the Raman signal, whereas the analogue and digital balancing allows shot noise limited detection. First imaging results with polystyrene (PS) and polymethylmethacrylate (PMMA) beads confirm the advantages of TICO sparse-sampling. We achieved a pixel dwell time as low as 35 μs for an image differentiating both species. The mechanical properties of the applied voice coil stage for scanning the sample currently limits even faster acquisition.

Proceedings Article | 7 March 2017 Presentation + Paper

Presentation + Paper

Pulse-to-pulse wavelength switching of diode based fiber laser for multi-color multi-photon imaging

Matthias Eibl, Sebastian Karpf, Hubertus Hakert, Daniel Weng, Torben Blömker, Robert Huber

Proceedings Volume 10083, 100831C (2017) https://doi.org/10.1117/12.2251965

KEYWORDS: Fiber amplifiers, Fiber lasers, Two photon imaging, Semiconductor lasers, Raman scattering, Laser sources, Diodes, Amplifiers, Modulation, Switching, Raman spectroscopy, Picosecond phenomena, Switches, Four wave mixing

Read Abstract +

Proceedings Article | 21 February 2017 Presentation + Paper

Presentation + Paper

Two-photon-excited fluorescence (TPEF) and fluorescence lifetime imaging (FLIM) with sub-nanosecond pulses and a high analog bandwidth signal detection

Matthias Eibl, Sebastian Karpf, Hubertus Hakert, Daniel Weng, Robert Huber

Proceedings Volume 10069, 100691F (2017) https://doi.org/10.1117/12.2250831

KEYWORDS: Fiber lasers, Fiber amplifiers, Semiconductor lasers, Two photon excitation microscopy, Laser sources, Luminescence, Two photon imaging, Microscopy, Fluorescence lifetime imaging, Analog electronics, Signal detection, Photons, Data acquisition, Picosecond phenomena, Microscopes

Read Abstract +

Proceedings Article | 28 June 2016 Presentation

Presentation

4D megahertz optical coherence tomography (OCT): imaging and live display beyond 1 gigavoxel/sec (Conference Presentation)

Robert Huber, Wolfgang Draxinger, Wolfgang Wieser, Jan Philip Kolb, Tom Pfeiffer, Sebastian Karpf, Matthias Eibl, Thomas Klein

Proceedings Volume 9720, 97200M (2016) https://doi.org/10.1117/12.2212498

KEYWORDS: Optical coherence tomography, Coherence imaging, Tissues, Imaging systems, Diagnostics, Ophthalmology, Cardiology, Image resolution, In vivo imaging, Microscopes

Read Abstract +

Proceedings Article | 14 July 2015 Paper

Paper

Nanosecond two-photon excitation fluorescence imaging with a multi color fiber MOPA laser

Sebastian Karpf, Matthias Eibl, Robert Huber

Proceedings Volume 9536, 953616 (2015) https://doi.org/10.1117/12.2183854

KEYWORDS: Imaging systems, Fiber lasers, Luminescence, Picosecond phenomena, Raman spectroscopy, Microscopy, Two photon imaging, Fiber amplifiers, Modulation, Biomedical optics

Read Abstract +

Proceedings Article | 14 July 2015 Paper

Paper

Hyperspectral stimulated Raman microscopy with two fiber laser sources

Matthias Eibl, Sebastian Karpf, Wolfgang Wieser, Thomas Klein, Robert Huber

Proceedings Volume 9536, 953604 (2015) https://doi.org/10.1117/12.2183822

KEYWORDS: Raman spectroscopy, Fiber lasers, Laser beam diagnostics, Microscopy, Pulsed laser operation, Modulation, Semiconductor lasers, Fiber amplifiers, Imaging systems, Oscillators

Read Abstract +

Proceedings Article | 9 July 2015 Paper

Paper

Time-encoded Raman scattering (TICO-Raman) with Fourier domain mode locked (FDML) lasers

Sebastian Karpf, Matthias Eibl, Wolfgang Wieser, Thomas Klein, Robert Huber

Proceedings Volume 9541, 95410F (2015) https://doi.org/10.1117/12.2183859

KEYWORDS: Raman spectroscopy, Fiber lasers, Pulsed laser operation, Laser scattering, Microscopy, Optical coherence tomography, Laser beam diagnostics, Mode locking, Imaging systems, Raman scattering

Read Abstract +

Showing 5 of 23 publications

SIGN IN TO:

View contact details

UPDATE YOUR PROFILE

Is this your profile? Update it now.

Sign into your SPIE.org account

Don’t have a profile and want one?

Create an account on SPIE.org

Advertisement

Advertisement