The potential for the use of biological agents by terrorists is a real threat. Two approaches for antibody-based detection
of biological species are described in this paper: 1) The use of microbead arrays for multiplexed flow cytometry
detection of cytokines and botulinum neurotoxin simulant, and 2) a microfluidic platform for capture and separation of
different size superparamagnetic nanoparticles followed by on-chip fluorescence detection of the sandwich complex.
These approaches both involve the use of automated fluidic systems for trapping antibody-functionalized microbeads,
which allows sample, assay reagents, and wash solutions to be perfused over a micro-column of beads, resulting in faster
and more sensitive immunoassays. The automated fluidic approach resulted in up to five-fold improvements in
immunoassay sensitivity/speed as compared to identical immunoassays performed in a typical manual batch mode. A
second approach for implementing multiplexed bead-based immunoassays without using flow cytometry detection is
currently under development. The goal of the microfluidic-based approach is to achieve rapid (<20 minutes),
multiplexed (≥ 3 bioagents) detection using a simple and low-cost, integrated microfluidic/optical detection platform.
Using fiber-optic guided laser-induced fluorescence, assay detection limits were shown to be in the 100's of picomolar
range (10's of micrograms per liter) for botulinum neurotoxin simulant without any optimization of the microfluidic
device or optical detection approach.
Conference Committee Involvement (6)
Advances in Global Health through Sensing Technologies 2015
20 April 2015 | Baltimore, MD, United States
Sensing Technologies for Global Health, Military Medicine, and Environmental Monitoring IV
5 May 2014 | Baltimore, MD, United States
Sensing Technologies for Global Health, Military Medicine, and Environmental Monitoring III
29 April 2013 | Baltimore, Maryland, United States
Sensing Technologies for Global Health, Military Medicine, Disaster Response, and Environmental Monitoring II
23 April 2012 | Baltimore, Maryland, United States
Sensing Technologies for Global Health, Military Medicine, Disaster Response, and Environmental Monitoring
25 April 2011 | Orlando, Florida, United States
Sensors, and Command, Control, Communications, and Intelligence (C3I) Technologies for Homeland Security and Homeland Defense IX
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