An optical system for measurement of the intracellular pH (pHi) in a single living cell by using the fluorescent probe, 5(6)-carboxyfluorescein diacetate (CFDA) and fiber optic nanoprobe was demonstrated in this work. The CFDA probe is used to determine pHi in the yeast, Saccharomyces cerevisiae 97 while fiber optic nanoprobe is used to guide excitation light and receive emission light within a single cell. Experimental results showed that our system had higher detection sensitivity than other standard spectrometer, which is important to single-cell analysis, especially for the microanalysis in a single-cell.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.