Ms. Qi Zhao Profile

Qi Zhao

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Proceedings Article | 30 April 2024 Paper

Detecting intracellular onion-like carbon nanostructures based on cell biolens’ properties by holographic flow cytometry

Yijing Li, Yakun Liu, Yuchen Feng, Qi Zhao, Xi Xiao, Hao Wang, Wen Xiao, Feng Pan

Proceedings Volume 13157, 1315705 (2024) https://doi.org/10.1117/12.3013239

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In recent years, onion-like carbons (OLCs), as a new type of carbon nanostructure, have emerged as significant in biomedical fields. OLCs are capable of being internalized by cells, interacting with various organelles, and thereby influencing cellular physiological processes. Consequently, there is considerable interest in the rapid, non-invasive detection and statistical analysis of intracellular OLCs. Holographic flow cytometry provides a high-throughput, label-free imaging approach, introducing a new approach for detecting intracellular OLCs. Indeed, cells can act as biological lenses, and the presence of intracellular OLCs alters their refractive index distribution, affecting their optical focusing and lensing features. In this study, we merged the 3D refractive index distribution of cells, obtained through in-flow tomographic experiments, with appropriate numerical simulations. This combination demonstrates that intracellular OLCs can be effectively detected by analyzing 2D quantitative phase maps, without the need for additional manual labeling. The experiments were conducted on colon cancer cells, both with and without intracellular OLCs. The results indicate that the biolens properties of cells can serve as a valuable biomarker for detecting intracellular OLCs. This promotes the research on OLCs-related physiological processes using holographic flow cytometry, enabling high-throughput, non-invasive screening of statistically significant number of cells.

Proceedings Article | 11 August 2023 Poster + Paper

Three-dimensional distribution dynamic change observation of carbon nano-onions particle inside cancer cell based on digital holographic tomography

Yakun Liu, Wen Xiao, Xiaosu Yi, Xi Xiao, Hao Wang, Ran Peng, Yuchen Feng, Qi Zhao, Feng Pan

Proceedings Volume 12622, 126221B (2023) https://doi.org/10.1117/12.2676365

KEYWORDS: Cancer, Nanoparticles

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Cancer remains a significant global medical challenge, and the selection of effective treatment modalities is crucial for an optimistic prognosis. Photothermal therapy, being non-invasive and targeted, holds immense potential for future therapeutic developments. Due to their high biocompatibility, carbon nano-onions particles are frequently employed as photothermal materials. The investigation of the dynamic three-dimensional distribution of these nanoparticles within cancer cells is imperative for constructing an accurate photothermal conversion model. In this research, we employed digital holographic tomography to monitor the temporal changes in the three-dimensional distribution of onion-like carbon nanoparticles within colorectal cancer cells. We reconstructed the three-dimensional refractive index distribution of carbon nano-onions particles within cancer cells at different time points. Further, we quantified two morphological parameters, surface area and volume, of these nanoparticles within cancer cells and performed preliminary analysis of their temporal evolution. This methodology introduces a novel perspective to study the interaction between Carbon nano-onions particles and cancer cells, enhancing our understanding of the photothermal therapy mechanism.

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