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In this paper we consider the approach described for the treatment of fluorescence measurements of dissolved organic matter and chlorophyll-a in seawater. Joint analysis of the various pairs of wavelengths of excitation / emission fluorescence, fluorescence analysis at different durations of the exciting radiation and the time-spatial analysis of the received signal allow identifying different types of fluorescent dissolved organic matter and estimate their stage of biodegradation, to study the functional state of phytoplankton cells. So it is possible to provide real-time investigation of environmental indicators of seawater.
One of the main limitations of the EEM is that usually necessary to carry out research in the laboratory on the specialized equipment. The paper describes the hardware system allowing carry out measurements in the field studies.
The hardware system includes a set of light-emitted diodes (LEDs) working in the range from 245 to 600 nm, which produce sequential excitation fluorescence of the sample liquid, which is detected using a 32-channel photomultiplier tube (PMT). Number of LEDs can vary from 7 to 16. Through the use of multi-channel photomultiplier reached the required measurement sensitivity and efficiency measurement is less than 1 minute for full cycle.
Minimum 4 channel in excitation range of 350-600 nm support the work of variable duration excitation pulses from microseconds to seconds. It makes possible to measure the fluorescence of the chlorophyll-a with closed or open reaction centers of phytoplankton cells. The use of multiple excitation radiation allows to activate various mechanisms of energy transfer in the photosynthetic apparatus of phytoplankton cells, improves measurement accuracy, reduces dependence on the variation in species composition and functional state of phytoplankton.
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