Ms. Osheen Joseph Profile

Osheen Joseph

Student at CUSAT

SPIE Involvement:

Author

Publications (1)

This will count as one of your downloads.

You will have access to both the presentation and article (if available).

DOWNLOAD NOW

This content is available for download via your institution's subscription. To access this item, please sign in to your personal account.

Email or Username Forgot your username?

Password Forgot your password?

Show

Keep me signed in

No SPIE account? Create an account

Proceedings Article | 7 October 2019 Paper

Nile red-dye based analysis of synthetic fibres for forensic applications

Sandhya Devalla, Osheen Joseph, Radhakrishna Prabhu

Proceedings Volume 11166, 111660Y (2019) https://doi.org/10.1117/12.2536780

KEYWORDS: Forensic science, Microscopy, Luminescence, Spectroscopes, Optical filters, Microscopes, Optical microscopy, Error analysis

Read Abstract +

Forensic evaluation of crime scenes normally involves examination of textile fibers, to find out the association between an individual and a crime scene, or between a suspect and a victim. The forensic samples normally include a mix of various types, sizes (micro to nano - scale) and shapes of natural and synthetic fibers, which are very difficult to differentiate/identify. Various sophisticated analytical instruments are being used to carry out the examination of these fibers. They involve various microscopy and spectroscopy based techniques, most of which are very complex and highly sensitive. Further, they may require a series of sample preparation steps to get high selectivity and are highly time consuming. Here we report a fluorescence microscopy based synthetic (plastic) fiber detection method using Nile Red (NR) dye, which provides high selectivity for synthetic fibers. The methodology involves the use of NR dye which selectively stains the fibers collected on filter papers following separation from samples/soils and water. The selectivity of NR towards the fibers is due to their non-polar property. Binding with NR makes the fibers fluoresce when viewed under a fluorescence microscope. This selectivity of NR for fibers makes the identification of fibers lot easier and less timeconsuming in forensic samples when compared to the more commonly used optical microscopy (where the presence of naturally-occurring substances of similar size can result in more errors). The paper will discuss optimisation of various parameters and method validation for detection of synthetic fibers and microplastics from soil samples. As an example, our method has shown to provide distinct clarity for the analysis of microfibers. The potential for the application of the method for faster forensics analysis will be discussed.

My Library

You currently do not have any folders to save your paper to! Create a new folder below.

Folder Name

Folder Description

View contact details

UPDATE YOUR PROFILE

Is this your profile? Update it now.

Sign into your SPIE.org account

Don’t have a profile and want one?

Create an account on SPIE.org

Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks. You are receiving this notice because your organization may not have SPIE eBooks access.*

*Shibboleth/Open Athens users─please sign in to access your institution's subscriptions.

To obtain this item, you may purchase the complete book in print or electronic format on SPIE.org.

ORGANIZATIONAL
Sign in with credentials provided by your organization.

Organizational Username

Organizational Password

Show/Hide Password

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members:

Non-members: ADD TO CART

Keywords/Phrases

Search In:

Publication Years