Imaging with third harmonic generation (THG) provides a powerful label-free approach to probing biological systems, particularly looking at structures formed by lipids. We present the first demonstration of THG widefield holographic microscopy. Holographic microscopy preserves the amplitude and phase of the measured field, which enables us to construct a synthetic spatial aperture with expanded field of view for complex valued THG images. Additionally, we implement a matrix approach postprocessing algorithm to reconstruct aberration-free THG holographic images by estimating and correcting for phase aberrations and scattering distortions.
Non-invasive imaging with high resolution deep within biological materials without the use of harmful ionizing radiation is of great interest in the field of medical imaging. Second harmonic generation is an excellent mechanism to circumvent this issue by providing outstanding contrast and optical sectioning. In general, these signals are weak and prone to scattering which introduce great challenges when imaging deep within turbid media. We will discuss recently demonstrated Epi-SHG holography, which can detect very weak backscattered SHG signals and enables the application of recently developed techniques which utilize the phase information to allow diffraction limited imaging within deep tissue.
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