The object of the study was an aqueous dispersion of gold nanoparticles stabilized with polyvinyl alcohol (AuPVA). It was previously found that AuPVA is characterized by a broad spectrum of antimicrobial activity against standard and clinical strains of gram-positive and gram-negative bacteria and yeasts. The safety of using AuPVA is associated with the absence of toxic effects of its working concentrations on biotest objects and cell cultures. Evaluation of the effectiveness of photodynamic antimicrobial therapy was carried out on a model of experimental full-sleep purulent wounds, which were formed in the interscapular region in rats. We used a semiconductor laser with a wavelength of 405 nm (manufactured by AlcomMedica, St. Petersburg). It was found that the use of 1% aqueous dispersion of AuPVA as a photosensitizer led to a 1,6-fold reduction in the wound healing time compared to the control.
The aim of the study was to assess the effectiveness of the original treatment of patients with psoriasis, including VLOK- 525 and the local impact of pulsed IR NEELIE. Under the supervision of a 264 patients with widespread forms of psoriasis in progresive stage (163 men and 101 women) between the ages of 24 to 63 years old (average age - 41 years), being diagnosted with the disease from 1 to 30 years ago. In the main group the complex treatment included the original laser methods apparatus " LAZMIK VLOK," with the laser head emit KL-VLOK-525-20 for intravenous laser blood irradiation and ML-635-40 for external impact. It was shown that combined laser therapy of patients with a moderate form of psoriasis (10 <PASI<50), including a local effect on psoriatic elements by pulsed red light LIL (635 nm) with a power of 40 W with a pulse repetition rate of up to 10 Hz OOO-VLOK-667 allows effectively the cease of inflammatory changes and lead to a reduction in erythema, infiltration and exfoliation.
The study of antimicrobial photodynamic activity of subingibing concentrations of aqueous solutions of silver nanoparticles under the photodynamic action of blue led radiation in relation to the standard and clinical strain of Staphylococcus aureus. The greatest sensitivity to the combined effects of radiation and metal nanoparticles showed methicillin-resistant clinical strain of Staphylococcus aureus. The samples containing silver nanoparticles presented in the experiment were characterized by a pronounced antimicrobial activity and can be considered as promising photosensitizers for photodynamic therapy of pyoinflammatory diseases.
KEYWORDS: Biomedical optics, Medical devices, Pathogens, Bacteria, In vitro testing, Scanning electron microscopy, Microorganisms, Crystals, Medical research, Electron microscopy
The current study was designed to investigate the dynamics of microbial biofilm-formation in standard and clinical strains of uropathogenic Escherichia coli (UPEC), using spectrophotometric and electron microscopic methods. For achieving this goal, three E. coli strains were used (one standard and two clinical). These isolates differ in the existence of fimH virulence gene. The process involved the using of electron microscopy and immunological microtitration plates under in vitro conditions for 96 hours. We found that standard and clinical strains of E. coli were capable of forming a microbial biofilm in vitro, with a higher intensity related to the clinical strain E. coli 245. The using of scanning electron microscopy confirmed the obtained results. The combined use of different optical methods gives a complete characterization of film-forming ability of pathogenic microorganisms.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.