Nanoparticle probes for use in targeted detection schemes and readout by surface-enhanced Raman scattering (SERS)
comprise a metal core, Raman reporter molecules and a protective shell. One design of SERS labels specifically
optimized for biomedical applications in conjunction with red laser excitation is based on tunable gold/silver nanoshells,
which are completely covered by a self-assembled monolayer (SAM) of Raman reporters. A shell around the SAM-coated
metal core stabilizes the colloid and prevents particle aggregation. The optical properties and SERS efficiencies
of these plasmonic nanostructures are characterized both experimentally and theoretically. Subsequent bioconjugation of
SERS probes to ligands such as antibodies is a prerequisite for the selective detection of the corresponding target
molecule via the characteristic Raman signature of the label. Biomedical imaging applications of SERS-labeled
antibodies for tumor diagnostics by SERS microscopy are presented, using the localization of the tumor suppressor p63
in prostate tissue sections as an example.
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