We propose a new toolset to characterize the role of corneal nerves in ocular conditions such as dry eye disease (DED). By automating analysis (nerve segmentation, density, detection of whorl patterns and nerve beading) we quantify the nerve structure over whole ex vivo corneas in a mouse model of evaporative DED. We also created a mouse line of DED with genetically encoded calcium markers and successfully imaged neural activity over time in live corneas. This combined structural and functional quantitative approach will further our understanding of corneal nerves in disease and accelerate the development of novel therapies.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.