Administration of 5-aminolevulinic acid (ALA) as a precursor for protoporphyrin IX (PpIX) is an established photosensitization strategy for photodynamic therapy (PDT) and fluorescence guided surgery. Ongoing studies seek to identify strategies to enhance PpIX accumulation as well as phenotypic traits that correlate with high PpIX conversion. Here we examine PpIX accumulation, PDT response, and differential gene expression in pancreatic and oral cancer cells compared to derivative sublines with acquired drug resistance. The latter exhibit poor differentiation and increased EMT but marked increase in PpIX conversion and enhanced response to PDT. RNAseq analysis shows dramatic changes in expression of genes relevant to PpIX transport (ABCG2) and PpIX formation (ALAD).
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