We previously proposed a three-dimensional microscopic imaging system for objects hidden behind scattering media using in-line phase-shift digital holography, which simultaneously captures amplitude and phase information. However, as the thickness of the scattering medium increases, the influence of scattering is enhanced, and the reconstructed image of the object behind the scattering medium deteriorates. In this paper, we report the evaluation of this image using a near-infrared light source with a wavelength of 780 nm that is capable of deep tissue penetration. A favorable microscopic image of the object behind the rat-skin sample of 912 μm-thickness was successfully reconstructed.
In recent years, noninvasive, nonstaining, and nondestructive quantitative cell measurement techniques have become increasingly important in the medical field. These cell measurement techniques enable the quantitative analysis of living cells, and are therefore applied to various cell identification processes, such as those determining the passage number limit during cell culturing in regenerative medicine. To enable cell measurement, we developed a quantitative microscopic phase imaging system based on a Mach–Zehnder interferometer that measures the optical path length difference distribution without phase unwrapping using optical phase locking. The applicability of our phase imaging system was demonstrated by successful identification of breast cancer cells amongst normal cells. However, the cell identification method using this phase imaging system exhibited a false identification rate of approximately 7%. In this study, we implemented a polarimetric imaging system by introducing a polarimetric module to one arm of the Mach–Zehnder interferometer of our conventional phase imaging system. This module was comprised of a quarter wave plate and a rotational polarizer on the illumination side of the sample, and a linear polarizer on the optical detector side. In addition, we developed correction methods for the measurement errors of the optical path length and birefringence phase differences that arose through the influence of elements other than cells, such as the Petri dish. As the Petri dish holding the fluid specimens was transparent, it did not affect the amplitude information; however, the optical path length and birefringence phase differences were affected. Therefore, we proposed correction of the optical path length and birefringence phase for the influence of elements other than cells, as a prerequisite for obtaining highly precise phase and polarimetric images.
We have proposed a digital holographic microscope using a planar lightwave circuit. Using the system, we report the evaluation of the spatial resolution and measurement of Closterium.
Automatic video identification is important for indexing, search purposes, and removing illegal material on the
Internet. By combining a high-speed correlation engine and web-scanning technology, we developed the Fast
Recognition Correlation system (FReCs), a video identification system for the Internet. FReCs is an application
thatsearches through a number of websites with user-generated content (UGC) and detects video content that violates
copyright law. In this paper, we describe the FReCs configuration and an approach to investigating UGC websites using
FReCs. The paper also illustrates the combination of FReCs with an optical correlation system, which is capable of
easily replacing a digital authorization sever in FReCs with optical correlation.
Using a holographic disc memory on which a huge amount of data can be stored, we constructed an ultra-high-speed,
all-optical correlation system. In this method, multiplex recording is, however, restricted to “one page” on “one spot.” In
addition, signal information must be normalized as data of the same size, even if the object data size is smaller. Therefore,
this system is difficult to apply to part of the object data scene (i.e., partial scene searching and template matching),
while maintaining high accessibility and programmability. In this paper, we develop a holographic correlation system by
a time division recording method that increases the number of multiplex recordings on the same spot. Assuming that a
four-channel detector is utilized, 15 parallel correlations are achieved by a time-division recording method. Preliminary
correlation experiments with the holographic optical disc setup are carried out by high correlation peaks at a rotational
speed of 300 rpm. We also describe the combination of an optical correlation system for copyright content management
that searches the Internet and detects illegal contents on video sharing websites.
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