Mr. Joshua W. Su Profile

Joshua W. Su

at Nanyang Technological Univ

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Proceedings Article | 19 July 2019 Paper

Depth sensitive Raman spectroscopy for skin wounds in rodents

Joshua Su, Qiang Wang, Yao Tian, Leigh Madden, Erica M. Teo, David Becker, Quan Liu

Proceedings Volume 11073, 110731C (2019) https://doi.org/10.1117/12.2526556

KEYWORDS: Skin, Raman spectroscopy, Wound healing, Axicons, Tissue optics, Biomedical optics, Light scattering, Raman scattering

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Raman spectroscopy has demonstrated its great potential in skin wound assessment. Given that biochemical changes in skin wound healing is a layer dependent process, depth sensitive Raman spectroscopy could enhance the power of Raman spectroscopy in this application. Considering the critical importance of rodent studies in the field of skin wound assessment, it is necessary to develop and validate a system that can perform depth sensitive measurements in rat skin with a proper target depth range. In this manuscript, we report the design, optimization and evaluation of a new snapshot depth-sensitive Raman instrument for rat skin measurements. The optical design and optimization process are presented first. The depth sensitive measurement performance is characterized on ex vivo rat skin samples with wounds. Raman signal emitted by the ex vivo rat skin from different target depths were simultaneously acquired. The feasibility of using the measured Raman spectra to differentiate between the wound edge and healthy skin was validated using PLS-LDA with leave-one-out. The accuracy of the classification improves monotonically as more data from new depths are used, which implies that each depth offers additional information useful for classification. This instrument demonstrates the ability to perform snapshot depth sensitive Raman measurements from rat skin, which paves the way towards in vivo preclinical studies of rat skin wounds.

Proceedings Article | 28 July 2017 Paper

Efficiency enhancement of Raman microspectroscopy at long working distance by parabolic reflector

Yao Tian, Joshua Weiming Su, Jian Ju, Quan Liu

Proceedings Volume 10411, 1041103 (2017) https://doi.org/10.1117/12.2281826

KEYWORDS: Raman spectroscopy, Reflectors, Luminescence

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Raman microspectroscopy is well suited for readily revealing information about bio-samples. As such, this technique has been applied to a wide range of areas, especially in bio-medical diagnosis. However, bio-samples typically suffer from low Raman signal level due to the nature of inelastic scattering of photons, To achieve a decent signal level, usually a high numerical aperture is employed. One drawback with these objectives is that their working distance is very short. In many cases of clinic diagnosis, a long working distance is always desired which limits the usage of these objectives. We propose a practical solution to this problem by enhancing the Raman/fluorescence signal by a parabolic reflector. On one hand, the high signal level is achieved by the large solid angle of collection of the parabolic reflector. On the other hand, the long working distance is guaranteed by the novel design of our microscope. The enhancement-capability is demonstrated through five types of samples among which we found the method is most applicable for turbid samples.

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