Induced pluripotent stem cells (iPSCs) hold the potential for personalized regenerative medicine. Yet accurately gauging the stemness of each colony remains a challenge since existing methods are inconsistent or harmful to iPSCs. Addressing this, we introduce holotomography (HT), a non-invasive microscopic technique. HT, through three-dimensional refractive index distributions, revealed iPSC structures at various scales as well as properties like volume, mass density, and lipid ratio. We identified altered properties in iPSCs exposed to differentiation agents, and then employed a machine-learning algorithm to detect reduced stemness from images. Through these results, HT emerges as a potential tool for iPSC quality maintenance.
Accurate and rapid evaluation of dynamic immune status is critical to determine therapeutic modalities for sepsis patients, which is impeded by the limitations of conventional diagnostic tools. Here, we employ refractive index tomography to quantitatively assess the immune status of human monocytes in a label-free manner. Measurement of refractive index tomograms enabled quantifications of three-dimensional morphological parameters, which revealed a clear increment in lipid droplets content and intracellular inhomogeneities as the septic stage progresses. We leveraged these observations to engineer a deep-learning-based algorithm that predicts the immune status of monocytes, showing over 99 % blind test accuracy.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.