This paper presents the use of radial basis functions (RBF) for describing freeform optical
surfaces. The RBF approximation framework along with preliminary optical design experiences will
be summarized.
Optical Coherence Microscopy (OCM) utilizes a high NA microscope objective in the sample arm to achieve an axially
and laterally high resolution OCT image. An increase in NA, however, leads to a dramatically decreased depth of focus
(DOF), and hence shortens the imaging depth range so that high lateral resolution is maintained only within a small
depth region around the focal plane. One solution to increase the depth of imaging while keeping a high lateral resolution
is dynamic-focusing. Utilizing the voltage controlled refocus capability of a liquid lens, we have recently presented a
solution for invariant high resolution imaging using the liquid lens embedded within a fixed optics hand-held custom
microscope designed specifically for optical imaging systems using a broadband light source at 800 nm center
wavelength. Subsequently, we have developed a Gabor-Domain Optical Coherence Microscopy (GD-OCM) that utilizes
the high speed imaging of spectral domain OCT, the high lateral resolution of OCM, and the ability of real time
refocusing of our custom design variable focus objective. In this paper we demonstrate in detail how portions of the infocus
cross-sectional images can be extracted and fused to form an invariant lateral resolution image with multiple crosssectional
images acquired corresponding to a discrete refocusing step along depth enabled by the varifocal probe. We demonstrate sub-cellular resolution imaging of an African frog tadpole (Xenopus Laevis) taken from a 500 μm x 500 μm cross-section.
In this paper, we present a developing technology targeted at clinical imaging, Gabor Domain Optical Coherence
Microscopy (GD-OCM), which combines the high resolution imaging of optical coherence microscopy (OCM), high
imaging speed of Fourier domain optical coherence tomography (FD-OCT), and invariant lateral resolution of our
custom designed dynamic focusing objective. A high lateral resolution optical design of a dynamic-focusing optical
probe with no moving parts, which provides an invariant resolution of currently < 3 μm across a 2mm full-field of view
and 2mm imaging depth, is presented. Furthermore, an acquisition scheme (using the probe) that is capable of
performing automatic real time data fusion to render an in-focus high resolution image throughout the depth of sample in
real time was implemented. 3D imaging of an African frog tadpole is demonstrated at cellular level resolution.
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