Now, spectra technology is widely used in the biomedicine research,so this study investigates variation of the
fluorescence spectra in different excitation wavelength, and the spectra of serum with different glucose concentration is
tested in the excitation wavelength of 240nm to 280nm. The experimental result shows that the correlation between the
serum fluorescence intensity and the excitation light is very close, when the excitation light is in the ultraviolet wave
band, the fluorescence of serum is intensive. There is a violent fluorescence emission wavelength, which is 300nm to
410nm, while the excitation wavelength ranges from 220nm to 290nm, and the peaks wavelength are 330nm and
370nm. From 240nm to 280nm, the serum fluorescence intensity increases synchronously with the glucose concentration,
and the relationship between the fluorescence peak wavelength and the glucose concentration is almost in line. In this
way the blood sugar concentration can be estimated by the fluorescence spectra peak wavelength when the excitation
wavelength is from 240nm to 280nm, which is effective. It provides experimental foundation for the wide use of spectra
technology in medical diagnose, and the effectiv method to test the blood sugar concentration.
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