Mr. Hoyoon Lee Profile

Hoyoon Lee

at Korea Univ

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Proceedings Article | 9 March 2016 Paper

Study of erythrocyte membrane fluctuation using light scattering analysis

Hoyoon Lee, Sangyun Lee, YongKeun Park, Sehyun Shin

Proceedings Volume 9718, 971818 (2016) https://doi.org/10.1117/12.2210777

KEYWORDS: Light scattering, Blood, Diagnostics, Spatial frequencies, Statistical analysis, Diffraction, Image sensors, Picosecond phenomena, Scattering, Biomedical optics

Read Abstract +

It is commonly known that alteration of erythrocyte deformability lead to serious microcirculatory diseases such as retinopathy, nephropathy, etc. Various methods and technologies have been developed to diagnose such membrane properties of erythrocytes. In this study, we developed an innovative method to measure hemorheological characteristics of the erythrocyte membrane using a light scattering analysis with simplified optic setting and multi-cell analysis as well. Light scattering intensity through multiple erythrocytes and its power density spectrum were obtained. The results of light scattering analyses were compared in healthy control and artificially hardened sample which was treated with glutaraldehyde. These results were further compared with conventional assays to measure deformable property in hemorheology. We found that light scattering information would reflect the disturbance of membrane fluctuation in artificially damaged erythrocytes. Therefore, measuring fluctuation of erythrocyte membrane using light scattering signal could facilitate simple and precise diagnose of pathological state on erythrocyte as well as related complications.

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