Chemically functionalized semiconductor quantum dot protocols were optimized for the specific labeling and imaging of
neural cells, both neurons and macroglial cells. Beta-tubulin III was used to image primary cortical neurons and PC12
cells while glial fibrillary acidic protein (GFAP) was used to image primary spinal cord and cortical astrocytes and the
rMC-1 retinal glial Muller cell line. Both proteins are the main components of intermediate filaments and are specific to
the two classes of neural cells. We also specifically labeled and imaged at high resolutions using anti-GFAP conjugated
quantum dots glial scars in situ in intact neural sensory retina in a rodent model of macular degeneration.
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