Dr. DongKyun Kang Profile

Dr. DongKyun Kang

Associate Professor at Wyant College of Optical Sciences

SPIE Involvement:

Conference Program Committee | Author

Publications (25)

SPIE Journal Paper | 6 August 2024 Open Access

Compact scattering-based light sheet microscopy probe using a custom miniature objective lens

Jingwei Zhao, Yongjun Kim, Momoka Sugimura, Kenneth Marcelino, Rafael Romero, Brooke Liang, Michelle Khan, Eric Yang, Dongkyun Kang

JOM, Vol. 4, Issue 03, 034501, (August 2024) https://doi.org/10.1117/12.10.1117/1.JOM.4.3.034501

KEYWORDS: Tissues, Objectives, Image resolution, Light sources and illumination, Microscopy, Visualization, Lenses, In vivo imaging, Point spread functions, Optical microsystems

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Proceedings Article | 13 March 2024 Poster

Through focus point spread function and modulus transfer function of intraocular lenses

Paige Sawyers, Jim Schwiegerling, Dongkyun Kang, Travis Sawyer

Proceedings Volume PC12824, PC1282418 (2024) https://doi.org/10.1117/12.2692798

KEYWORDS: Modulation transfer functions, Point spread functions, Intraocular lenses, Lenses, Image quality, Tolerancing, Surgery, Light sources, Lens design, Imaging systems

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Proceedings Article | 17 March 2023 Presentation

Smartphone-compatible confocal endomicroscope for imaging cervix in vivo

Nachiket Kulkarni, Andrew Masciola, Abhinav Nishant, Kyung-Jo Kim, Arthur Gmitro, Esther Freeman, Philippa Makanga, Aggrey Semeere, Miriam Nakelembe, DongKyun Kang

Proceedings Volume PC12356, PC1235601 (2023) https://doi.org/10.1117/12.2647921

KEYWORDS: Confocal microscopy, In vivo imaging, Cervix, Visualization, Endomicroscopy, Tissues, Spectroscopy, Quantitative analysis, Objectives, Light scattering

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Proceedings Article | 7 March 2022 Presentation

Axial spectral encoding for low-cost confocal endomicroscopy

Nachiket Kulkarni, Andrew Masciola, Abhinav Nishant, Kyungjo Kim, Arthur Gmitro, Esther Freeman, Aggrey Semeere, Miriam Nakalembe, DongKyun Kang

Proceedings Volume PC11937, PC119370P (2022) https://doi.org/10.1117/12.2609006

KEYWORDS: Confocal microscopy, Computer programming, Endomicroscopy, Tissue optics, Tissues, Light sources, Visualization, Spectroscopy, Prototyping, Objectives

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Proceedings Article | 3 December 2021 Paper

Low-cost chromatic confocal endomicroscope for the diagnosis of cervical precancer

Nachiket Kulkarni, Andrew Masciola, Abhinav Nishant, Kyungjo Kim, Arthur Gmitro, Esther Freeman, Aggrey Semeere, Miriam Nakelembe, DongKyun Kang

Proceedings Volume 11921, 119210E (2021) https://doi.org/10.1117/12.2615465

KEYWORDS: Confocal microscopy, Colorimetry, Objectives, Tissue optics, Collimation, Visualization, Image visualization, Image resolution, Tissues, Prototyping

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Proceedings Article | 5 March 2021 Presentation

Deep learning-based denoising in spectrally-encoded confocal microscopy

Jingwei Zhao, Manu Jain, Ucalene Harris, Kivanc Kose, Clara Curiel-Lewandrowski, Dongkyun Kang

Proceedings Volume 11620, 1162004 (2021) https://doi.org/10.1117/12.2577321

KEYWORDS: Confocal microscopy, Signal to noise ratio, Denoising, Skin, CMOS sensors, Microscopy, Microscopes, Interference (communication), Image restoration

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Proceedings Article | 22 July 2019 Paper

Smartphone-based epifluorescence microscope for fresh tissue imaging

Wenbin Zhu, Giacomo Pirovano, Cheng Gong, Nachiket Kulkarni, Christopher David Nguyen, Christian Brand, Thomas Reiner, Dongkyun Kang

Proceedings Volume 11075, 110750B (2019) https://doi.org/10.1117/12.2526857

KEYWORDS: Microscopes, Tissues, Microscopy, Tissue optics, Tumors, Point-of-care devices, Biopsy

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Proceedings Article | 7 March 2019 Presentation

Evaluating fibrin polymerization dynamics in three dimensions peri-operatively in cardiac surgical patients using spectrally encoded confocal microscopy (SECM) (Conference Presentation)

Diane Tshikudi, Olivier Simandoux, DongKyun Kang, Elizabeth Van Cott, Michael Andrawes, Dvir Yelin, Seemantini Nadkarni

Proceedings Volume 10855, 1085508 (2019) https://doi.org/10.1117/12.2510423

KEYWORDS: Polymerization, Surgery, Confocal microscopy, Fourier transforms, Blood, Video microscopy, Plasma, Video, Microscopy, Spatial resolution

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Proceedings Article | 4 March 2019 Presentation

Preclinical swine joint imaging using forward-viewing spectrally encoded endoscopy (Conference Presentation)

Adel Zeidan, Jiheun Ryu, Dukho Do, DongKyun Kang, Mitsuhiro Ikuta, Guillermo Tearney

Proceedings Volume 10854, 108540V (2019) https://doi.org/10.1117/12.2507889

KEYWORDS: Endoscopy, Preclinical imaging, Optical fibers, Computer programming, Surveillance, Optical design, Light wave propagation, Multimode fibers, Polishing, Detector arrays

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Proceedings Article | 14 March 2018 Presentation

Forward-viewing spectrally encoded endoscopy with angled detection optics (Conference Presentation)

Adel Zeidan, Dukho Do, DongKyun Kang, Mitsuhiro Ikuta, Guillermo Tearney

Proceedings Volume 10470, 104700S (2018) https://doi.org/10.1117/12.2285202

KEYWORDS: Endoscopy, Optical fibers, Computer programming, Surveillance, Optical design, Light wave propagation, Multimode fibers, Polishing, Detector arrays, Spectroscopy

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Proceedings Article | 19 April 2017 Presentation

Tethered SECM endoscopic capsule for the diagnosis of eosinophilic esophagitis (Conference Presentation)

Dukho Do, DongKyun Kang, Nima Tabatabaei, Catriona Grant, Norman Nishioka, Mireille Rosenberg, Paul Hesterberg, Qian Yuan, John Garber, Aubrey Katz, Wayne Shreffler, Guillermo Tearney

Proceedings Volume 10040, 100400G (2017) https://doi.org/10.1117/12.2253401

KEYWORDS: Endoscopy, Confocal microscopy, Esophagus, Biopsy, Visualization, Speckle, Gold, Image quality standards, Image resolution, Prototyping

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Eosinophilic Esophagitis (EoE) is an inflammatory disease caused by inhaled or ingested food allergies, and characterized by the infiltration of eosinophils in the esophagus. The gold standard for diagnosing EoE is to conduct endoscopy and obtain multiple biopsy specimens from different portions of the esophagus; an exam is considered positive if more than 15 eosinophils per high power field (HPF) in any of the biopsies. This method of diagnosis is problematic because endoscopic biopsy is expensive and poorly tolerated and the esophageal eosinophil burden needs to be monitored frequently during the course of the disease. Spectrally encoded confocal microscopy (SECM) is a high-speed confocal microscopy technology that can visualize individual eosinophils in large microscopic images of the human esophagus, equivalent to more than 30,000 HPF. Previously, we have demonstrated that tethered capsule SECM can be conducted in unsedated subjects with diagnosed EoE. However, speckle noise and the relatively low resolution in images obtained with the first capsule prototypes made it challenging to distinguish eosinophils from other cells. In this work, we present a next-generation tethered SECM capsule, which has been modified to significantly improve image quality. First, we substituted the single mode fiber with a dual-clad fiber to reduce speckle noise. A gradient-index multimode fiber was fusion spliced at the tip of the dual-clad fiber to increase the effective numerical aperture of the fiber from 0.09 to 0.15, expanding the beam more rapidly to increase the illumination aperture at the objective. These modifications enabled the new SECM capsule to achieve a lateral resolution of 1.8 µm and an axial resolution of 16.1 µm, which substantially improves the capacity of this probe to visualize cellular features in human tissue. The total size of the SECM capsule remained 6.75 mm in diameter and 31 mm in length. We are now in the process of testing this new SECM capsule in humans. Early results using this new SECM capsule suggest that this technology has the potential to be an effective tool for the diagnosis of EoE.

Proceedings Article | 3 May 2016 Presentation

Clinical experience using the tethered capsule-based spectrally encoded confocal microendoscopy for diagnosis of eosinophilic esophagitis (Conference Presentation)

Dukho Do, Sanaz Alali, DongKyun Kang, Nima Tabatabaie, Weina Lu, Catriona Grant, Amna Soomro, Norman Nishioka, Mireille Rosenberg, Paul Hesterberg, Qian Yuan, John Garber, Aubrey Katz, Wayne Shreffler, Guillermo Tearney

Proceedings Volume 9691, 96910L (2016) https://doi.org/10.1117/12.2218559

KEYWORDS: Confocal microscopy, Photomedicine, Esophagus, Biopsy, Endoscopy, Scattering, Gold, Standards development, Reflectivity, Lutetium

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Proceedings Article | 27 April 2016 Presentation

SECM half-inch tethered endoscopic capsule (HITEC) for esophageal imaging (Conference Presentation)

DongKyun Kang, Minkyu Kim, Robert Carruth, Weina Lu, Tao Wu, Sanaz Alali, Dukho Do, Amna Soomro, Catriona Grant, Aubrey Tiernan, Mireille Rosenberg, Norman Nishioka, Guillermo Tearney

Proceedings Volume 9691, 96910M (2016) https://doi.org/10.1117/12.2208942

KEYWORDS: Endoscopy, Tissue optics, Esophagus, Confocal microscopy, Tissues, In vivo imaging, Speckle, Lens design, Optics manufacturing, Endomicroscopy

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Proceedings Article | 27 April 2016 Presentation

Simple, monolithic optical element for forward-viewing spectrally encoded endoscopy (Conference Presentation)

Dukho Do, Dongkyun Kang, Mitsuhiro Ikuta, Guillermo Tearney

Proceedings Volume 9691, 96910O (2016) https://doi.org/10.1117/12.2213394

KEYWORDS: Optical components, Endoscopy, Optical design, Mirrors, Photomedicine, Image processing software, Diffraction gratings, Image resolution, Endoscopes, Medical imaging applications

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Proceedings Article | 26 April 2016 Presentation

Spectrally encoded confocal microscopy (SECM) for rapid assessment of breast excision specimens (Conference Presentation)

Elena Brachtel, Nicole Johnson, Amelia Huck, Travis Rice-Stitt, Mark Vangel, Barbara Smith, Guillermo Tearney, DongKyun Kang

Proceedings Volume 9703, 97030C (2016) https://doi.org/10.1117/12.2209699

KEYWORDS: Breast, Confocal microscopy, Surgery, Tissues, Diagnostics, Breast cancer, Image visualization, Tumors, Reflectivity, Natural surfaces

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SPIE Journal Paper | 11 June 2012 Open Access

Evaluation of optical reflectance techniques for imaging of alveolar structure

Carolin Unglert, Eman Namati, William Warger, Linbo Liu, Hongki Yoo, DongKyun Kang, Brett Bouma, Guillermo Tearney

JBO, Vol. 17, Issue 7, 071303, (June 2012) https://doi.org/10.1117/12.10.1117/1.JBO.17.7.071303

KEYWORDS: Lung, Tissues, Reflectivity, Imaging systems, Tissue optics, Image resolution, Visualization, In vivo imaging, Biomedical optics, Confocal microscopy

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Proceedings Article | 3 March 2009 Paper

Combined spectrally encoded confocal microscopy and optical frequency domain imaging system

DongKyun Kang, Melissa Suter, Caroline Boudoux, Patrick Yachimski, Brett Bouma, Norman Nishioka, Guillermo Tearney

Proceedings Volume 7172, 717206 (2009) https://doi.org/10.1117/12.808028

KEYWORDS: Imaging systems, Tissues, Biopsy, Confocal microscopy, Intestine, Image resolution, Objectives, Tissue optics, Esophagus, Mirrors

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Proceedings Article | 6 December 2005 Paper

Design of real-time confocal microscopy using spectral encoding technique and slit aperture

Jeongmin Kim, DongKyun Kang, DaeGab Gweon, YeongSoo Sohn, Hyungsuck Cho

Proceedings Volume 6049, 604906 (2005) https://doi.org/10.1117/12.647953

KEYWORDS: Confocal microscopy, Diffraction gratings, Confocal optics, Imaging systems, Computer programming, Objectives, Relays, Optical design, Charge-coupled devices, Polarizers

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Proceedings Article | 24 March 2005 Paper

Lateral resolution enhancement in confocal self-interference microscopy

DongKyun Kang, DaeGab Gweon

Proceedings Volume 5701, (2005) https://doi.org/10.1117/12.589526

KEYWORDS: Confocal microscopy, Resolution enhancement technologies, Microscopy, Point spread functions, Calcite, Objectives, Polarization, Birefringence, Diffraction, Industrial metrology

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Proceedings Article | 13 July 2004 Paper

Design and performance evaluation of reflection confocal microscopy using acousto-optical deflector and slit detector

SeugnWoo Lee, Dong-Kyun Kang, HongKi Yoo, Dae-Gab Gweon, Suk-Won Lee, Kwang-Soo Kim

Proceedings Volume 5324, (2004) https://doi.org/10.1117/12.529884

KEYWORDS: Confocal microscopy, Objectives, Mirrors, Relays, Optical design, Lens design, Diffraction, Image acquisition, Sensors, Scanning electron microscopy

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Proceedings Article | 13 July 2004 Paper

Error analysis and tolerance allocation for confocal scanning microscopy using the Monte Carlo method

Hongki Yoo, Dong-Kyun Kang, SeungWoo Lee, Junhee Lee, Dae-Gab Gweon

Proceedings Volume 5324, (2004) https://doi.org/10.1117/12.529942

KEYWORDS: Tolerancing, Confocal microscopy, Error analysis, Monte Carlo methods, Microscopy, Manufacturing, Assembly tolerances, Optics manufacturing, Optical components, Quality systems

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Proceedings Article | 20 September 2002 Paper

Reflecting optical system to increase signal intensity in confocal microscopy

Dong Kyun Kang, Jungwoo Seo, Dae gab Gweon

Proceedings Volume 4927, (2002) https://doi.org/10.1117/12.464066

KEYWORDS: Confocal microscopy, Optical transfer functions, Objectives, Signal detection, Point spread functions, Luminescence, Photodetectors, Contrast transfer function, Mirrors, Spherical lenses

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Proceedings Article | 17 September 2002 Paper

New focusing lens system and tolerance analysis for near-field recording system

Jun Hee Lee, Hyoung Kil Yoon, Dong Kyun Kang, Dae gab Gweon, Sang Tae Lee

Proceedings Volume 4930, (2002) https://doi.org/10.1117/12.483365

KEYWORDS: Tolerancing, Near field, Assembly tolerances, Optics manufacturing, Near field optics, Manufacturing, Solids, Objectives, Optical storage, Diffraction

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Proceedings Article | 4 October 2001 Paper

3D image reconstruction using optical sectioning in confocal scanning microscopy

Jungwoo Seo, Dong Kyun Kang, Sunglim Park, Dae gab Gweon

Proceedings Volume 4564, (2001) https://doi.org/10.1117/12.444112

KEYWORDS: Confocal microscopy, Adaptive optics, Microscopy, Sensors, Image enhancement, Objectives, Image quality, Mirrors, Signal detection, Microscopes

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Confocal scanning microscopy (CSM) has been used in biological application, materials science, semiconductor quality measurement and other non-destructive microscopic application. Small spot of light illuminates a sample, and a small detector that is ideally a point detector collects the reflected or transmitted light having the information of specimen. An image distribution can be reconstructed by a correlation analysis of spots with the high bandwidth. The mechanism for two-dimensional beam scanning and optical sectioning has an important role in CSM as the three-dimensional profiler. The parasitic motion of focus on the detector gives rise to the fatal distortion of an image profile named the extinction effect while using acousto-optical (AO) deflector. The intensity profile for the open loop scanning should be matched with its response for the standard. The non-linearity can be minimized with the optical sectioning or the optical probe of the closed loop control. This paper shows the mathematical expression of the light such as the extinction curve in the optical fields of system using AO deflector, the axial/lateral response experimentally when the error sources change, and the methods of optical sectioning. We propose the progressive methods for the high quality image as the following. At first, for having the corrected image, small spot and long scan range, this paper shows that the optimal design having the multi-objects can be used by choosing the unitary lens device in CSM. At second, in order to compensate for the intensity cancellation at the end profile that may be the cause of waviness for the optical image, this paper shows that it is efficient to schedule the frequency of scan. According to characteristics of the extinction curve and axial/lateral response having the error property, we can define the frequency and sensitivity of as their robustness. Finally, the axial response gives an important motive for the optical section, and the limit of object depth. The edge enhancement may be a fatal defeat to the reconstruction of image and sensitive to the conditions of specimen such as slope, irregular reflectivity, shape, etc. That means that the intensity profile for the open loop scanning method should be matched with its response to a perfect mirror as specimen, which can be minimized with the optical sectioning or the optical probe of the closed loop control.

Proceedings Article | 4 October 2001 Paper

Auto-alignment for incident angle of ellipsometer

Sunglim Park, Jaewha Jung, Jeongwoo Seo, Dong Kyun Kang, Dae gab Gweon

Proceedings Volume 4564, (2001) https://doi.org/10.1117/12.444104

KEYWORDS: Sensors, Signal detection, Algorithm development, Polarizers, Signal processing, Detection and tracking algorithms, Optical alignment, Manufacturing, Polarizing prisms, 3D modeling

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