Dr. Alyona Sukhanova Profile

Dr. Alyona Sukhanova

Researcher at Univ de Reims Champagne-Ardenne

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Publications (13)

Proceedings Article | 3 October 2019 Paper

Nanophotonic tools based on the conjugates of nanoparticles with the single-domain antibodies for multi-photon micrometastases detection and ultrasensitive biochemical assays

Alyona Sukhanova, Fernanda Ramos-Gomes, Patrick Chames, Daniel Baty, Frauke Alves, Pavel Samokhvalov, Igor Nabiev

Proceedings Volume 11207, 112072A (2019) https://doi.org/10.1117/12.2530233

KEYWORDS: Luminescence, Quantum dots, Tissues, Breast, Confocal microscopy, Tissue optics, Nanoprobes, Nanoparticles, Cancer, Nanophotonics

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Proceedings Article | 3 October 2019 Paper

Engineering of fluorescent biomaging tools for cancer cell targeting based on polyelectrolyte microcapsules encoded with quantum dots

Galina Nifontova, Maria Baryshnikova, Fernanda Ramos-Gomes, Frauke Alves, Igor Nabiev, Alyona Sukhanova

Proceedings Volume 11207, 112072D (2019) https://doi.org/10.1117/12.2530281

KEYWORDS: Cancer, Polymers, Carbonates, Calcium, Molecules, Quantum dots, Luminescence, Oncology, Computer programming, Adsorption

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Proceedings Article | 8 May 2014 Paper

Development and potential applications of microarrays based on fluorescent nanocrystal-encoded beads for multiplexed cancer diagnostics

Kristina Brazhnik, Regina Grinevich, Anton Efimov, Igor Nabiev, Alyona Sukhanova

Proceedings Volume 9129, 91292C (2014) https://doi.org/10.1117/12.2057825

KEYWORDS: Cancer, Proteins, Diagnostics, Silver, Flow cytometry, Prostate cancer, Luminescence, Multiplexing, Breast cancer, Molecules

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Advanced multiplexed assays have recently become an indispensable tool for clinical diagnostics. These techniques provide simultaneous quantitative determination of multiple biomolecules in a single sample quickly and accurately. The development of multiplex suspension arrays is currently of particular interest for clinical applications. Optical encoding of microparticles is the most available and easy-to-use technique. This technology uses fluorophores incorporated into microbeads to obtain individual optical codes. Fluorophore-encoded beads can be rapidly analyzed using classical flow cytometry or microfluidic techniques. We have developed a new generation of highly sensitive and specific diagnostic systems for detection of cancer antigens in human serum samples based on microbeads encoded with fluorescent quantum dots (QDs). The designed suspension microarray system was validated for quantitative detection of (1) free and total prostate specific antigen (PSA) in the serum of patients with prostate cancer and (2) carcinoembryonic antigen (CEA) and cancer antigen 15-3 (CA 15-3) in the serum of patients with breast cancer. The serum samples from healthy donors were used as a control. The antigen detection is based on the formation of an immune complex of a specific capture antibody (Ab), a target antigen (Ag), and a detector Ab on the surface of the encoded particles. The capture Ab is bound to the polymer shell of microbeads via an adapter molecule, for example, protein A. Protein A binds a monoclonal Ab in a highly oriented manner due to specific interaction with the Fc-region of the Ab molecule. Each antigen can be recognized and detected due to a specific microbead population carrying the unique fluorescent code. 100 and 231 serum samples from patients with different stages of prostate cancer and breast cancer, respectively, and those from healthy donors were examined using the designed suspension system. The data were validated by comparing with the results of the “gold standard” enzyme-linked immunosorbent assay (ELISA). They have shown that our approach is a good alternative to the diagnostics of cancer markers using conventional assays, especially in early diagnostic applications.

Proceedings Article | 24 October 2012 Paper

Semiconductor quantum dots affect fluidity of purple membrane from Halobacterium salinarum through disruption of bacteriorhodopsin trimer organization

Nicolas Bouchonville, Michael Molinari, Anthony Le Cigne, Michel Troyon, Alyona Sukhanova, Igor Nabiev

Proceedings Volume 8548, 85480V (2012) https://doi.org/10.1117/12.946610

KEYWORDS: Promethium, Proteins, Absorption, Atomic force microscopy, Phase modulation, Quantum dots, Semiconductors, Dichroic materials, Crystals, Fourier transforms

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Proceedings Article | 24 October 2012 Paper

Bi-photon imaging and diagnostics using ultra-small diagnostic probes engineered from semiconductor nanocrystals and single-domain antibodies

Hilal Hafian, Alyona Sukhanova, Patrick Chames, Daniel Baty, Michel Pluot, Jacques H. Cohen, Igor Nabiev, Jean-Marc Millot

Proceedings Volume 8548, 85480R (2012) https://doi.org/10.1117/12.960493

KEYWORDS: Tissues, Diagnostics, Luminescence, Absorption, Nanoprobes, Semiconductors, Nanocrystals, Quantum dots, Proteins, Two photon imaging

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Proceedings Article | 24 October 2012 Paper

Quantum dots induce charge-specific amyloid-like fibrillation of insulin at physiological conditions

Alyona Sukhanova, Simon Poly, Anton Shemetov, Igor Nabiev

Proceedings Volume 8548, 85485F (2012) https://doi.org/10.1117/12.946606

KEYWORDS: Proteins, Nanoparticles, Luminescence, Quantum dots, In vivo imaging, Dichroic materials, Signal processing, Molecules, Sodium, Particles

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Proceedings Article | 11 October 2012 Paper

Novel cholesteric materials doped with CdSe/ZnS quantum dots with photo- and electrotunable circularly polarized emission

Konstantin Mochalov, Alexey Yu. Bobrovsky, Vladimir Oleinikov, Alyona Sukhanova, Anton Efimov, Valery Shibaev, Igor Nabiev

Proceedings Volume 8475, 847514 (2012) https://doi.org/10.1117/12.929729

KEYWORDS: Luminescence, Liquid crystals, Ultraviolet radiation, Quantum dots, Polarization, Polymers, Photon polarization, Electro optics, Transmittance, 3D image reconstruction

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Proceedings Article | 10 October 2012 Paper

Controlled FRET efficiency in nano-bio hybrid materials made from semiconductor quantum dots and bacteriorhodopsin

Nicolas Bouchonville, Anthony Le Cigne, Alyona Sukhanova, Marie-belle Saab, Michel Troyon, Michael Molinari, Igor Nabiev

Proceedings Volume 8460, 84600X (2012) https://doi.org/10.1117/12.930747

KEYWORDS: Fluorescence resonance energy transfer, Energy transfer, Data modeling, Luminescence, Phase modulation, Absorption, Promethium, Proteins, Energy efficiency, Quantum dots

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Proceedings Article | 10 October 2012 Paper

Biosensing with thermosensitive fluorescent quantum dot-containing polymer particles

Alla Generalova, Vladimir Oleinikov, Alyona Sukhanova, Mikhail Artemyev, Vitaly Zubov, Igor Nabiev

Proceedings Volume 8460, 84600Y (2012) https://doi.org/10.1117/12.931427

KEYWORDS: Particles, Luminescence, Polymers, Temperature metrology, Proteins, Biosensing, Composites, Adsorption, Semiconductors, Nanocrystals

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Proceedings Article | 10 October 2012 Paper

Extension of the spectral range of bacteriorhodopsin functional activity by energy transfer from quantum dots

Vladimir Oleinikov, Nicolas Bouchonville, Alyona Sukhanova, Michael Molinari, Svetlana Sizova, Konstantin Mochalov, Anton Chistyakov, Evgeniy Lukashev, Aliaksandra Rakovich, John Donegan, Igor Nabiev

Proceedings Volume 8464, 84640Z (2012) https://doi.org/10.1117/12.929764

KEYWORDS: Fluorescence resonance energy transfer, Promethium, Phase modulation, Quantum dots, Energy transfer, Solar energy, Nanocrystals, Molecules, Luminescence, Absorption

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Proceedings Article | 9 October 2012 Paper

Low-field magnetic circular dichroism in silver and gold colloidal nanoparticles of different sizes, shapes, and aggregation states

Mikhail Artemyev, Roman Krutokhvostov, Dmitry Melnikau, Vladimir Oleinikov, Alyona Sukhanova, Igor Nabiev

Proceedings Volume 8457, 845729 (2012) https://doi.org/10.1117/12.929860

KEYWORDS: Nanoparticles, Magnetism, Silver, Gold, Molybdenum, Absorption, Plasmonics, Signal attenuation, Dichroic materials, Metals

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Proceedings Article | 2 February 2012 Paper

Oriented conjugates of monoclonal and single-domain antibodies with quantum dots for flow cytometry and immunohistochemistry diagnostic applications

Alyona Sukhanova, Klervi Even-Desrumeaux, Jean-Marc Millot, Patrick Chames, Daniel Baty, Mikhail Artemyev, Vladimir Oleinikov, Jacques Cohen, Igor Nabiev

Proceedings Volume 8232, 82320T (2012) https://doi.org/10.1117/12.905896

KEYWORDS: Nanoprobes, Diagnostics, Quantum dots, Flow cytometry, Molecules, Biopsy, Semiconductors, Gold, Nanoparticles, Nanocrystals

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Proceedings Article | 20 May 2009 Paper

Energy transfer processes in semiconductor quantum dots: bacteriorhodopsin hybrid system

Aliaksandra Rakovich, Alyona Sukhanova, Nicolas Bouchonville, Michael Molinari, Michel Troyon, Jacques H. Cohen, Yury Rakovich, John Donegan, Igor Nabiev

Proceedings Volume 7366, 736620 (2009) https://doi.org/10.1117/12.821731

KEYWORDS: Luminescence, Nanocrystals, Proteins, Absorption, Energy transfer, Semiconductors, Quantum dots, Solar energy, Absorbance, Fluorescence correlation spectroscopy

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Showing 5 of 13 publications

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