Significance: The development of a technique allowing for non-invasive measurement of retinal blood flow (RBF) in humans is needed to understand many retinal vascular diseases (pathophysiology) and evaluate treatment with potential improvement of blood flow. Aim: We developed and validated an absolute laser Doppler velocimeter (LDV) based on an adaptive optical fundus camera that provides simultaneously high-definition images of the fundus vessels and absolute maximal red blood cells (RBCs) velocity to calculate the absolute RBF. Approach: This new absolute LDV is combined with the adaptive optics (AO) fundus camera (rtx1, Imagine Eyes©, Orsay, France) outside its optical wavefront correction path. A 4-s recording includes 40 images, each synchronized with two Doppler shift power spectra. Image analysis provides a vessel diameter close to the probing beam, and the velocity of the RBCs in the vessels are extracted from the Doppler spectral analysis. A combination of these values gives an average of the absolute RBF. Results: An in vitro experiment consisting of latex microspheres flowing in water through a glass capillary to simulate a blood vessel and in vivo measurements on six healthy humans was done to assess the device. In the in vitro experiment, the calculated flow varied between 1.75 and 25.9 μL / min and was highly correlated (r2 = 0.995) with the flow imposed by a syringe pump. In the in vivo experiment, the error between the flow in the parent vessel and the sum of the flow in the daughter vessels was between −11 % and 36% (mean ± sd, 5.7 ± 18.5 % ). RBF in the main temporal retinal veins of healthy subjects varied between 0.9 and 13.2 μL / min. Conclusions: The AO LDV prototype allows for the real-time measurement of absolute RBF derived from the retinal vessel diameter and the maximum RBCs velocity in that vessel. |
1.IntroductionThe inner retina is supplied by the retinal circulation, which is characterized by lower flow than the choroid, high-oxygen extraction, absence of any anastomosis, and autonomic innervation. The retinal blood flow (RBF) adaptation to the perfusion pressure is modulated by a fine autoregulation based on a balance between myogenic (via endothelium) and metabolic (via neurons and glia) mechanisms.1 Abnormal variations of RBF have been reported in a variety of ocular diseases including age-related macular degeneration2–4 and glaucoma,5 but also in systemic diseases such as diabetes6,7 and systemic hypertension.8–10 Comprehensive knowledge of RBF, essential for understanding the pathophysiology of ocular and systemic diseases, is also the key to the evaluation of therapeutic strategies.11 RBF depends on the vascular cross section and the velocity of red blood cells (RBC). Several techniques have been developed to estimate or to measure RBF in the human eye such as fluorescein angiography,12 laser Doppler velocimeter (LDV),13–17 Doppler optical coherence tomography,18–20 optical coherence angiography21,22 (OCT-A), and laser Doppler holography23 (LDH). Although OCT-A is able to show where blood is circulating, it does not estimate velocity of RBC and thus gives no information about the flow rate.24 LDH has the potential to evaluate the velocity within the vessel but mainly depends on the frame rate, which must be in the order of 100 kHz. Although the canon laser blood flowmeter17 systems allow for quantification of blood flow using one acquisition, the LDV systems developed so far allow for the independent acquisition of blood velocity and retinal vascular diameters.14–16,25,26 Simultaneous measurements of retinal vascular diameters and velocity are highly advisable due to physiological variability of these parameters. The measurement of blood vessel diameter is based on fundus images combined with image analysis using densitometry.27,28 The recent and innovative technology using adaptive optics (AO) aims at correcting low-order and high-order ocular aberrations, enhances performance of the optical systems, and allows for high-resolution imaging of retinal vessels. For instance, the rtx1 instrument provides in vivo retinal images with high lateral resolution ( per pixel) and a quantitative analysis of microvascular structures, especially the measurement of retinal arteriolar wall thickness.29 To overcome the limitations of the present technologies, we developed an approach based on simultaneous measurements of retinal vessel diameters using AO technology that provides 16-bit images29 and is able to measure the inner retinal vessel and blood velocity using bidirectional LDV. In this paper, we report in vitro and in vivo experiments investigating the validity of blood flow rate measured by this new prototype (aoLDV). 2.Materials and Methods2.1.Description of the Instrument2.1.1.Camera with adaptive opticsImages were obtained using the commercially available AO retinal camera,30,31 which measures and corrects wavefront aberrations with a 750-nm super luminescent diode source and an AO system operating in a closed loop. A fundus area (i.e., in emmetropic eyes) is illuminated at 840 nm by a light emitting diode with low temporal coherence, and a stack of 40 fundus images is acquired in 4 s (10 images per second) by a charge-coupled device camera. The rtx1 provides a continuous fundus image that is used to align the probing beam. Its intensity is at the cornea, well below the maximum permissible exposure of at that wavelength (ANSI Z136.1-2000). 2.1.2.Principle of bidirectional laser Doppler velocimetryThe principle of absolute LDV for the human eye was first published by Riva et al.14 One probing beam with direction is focused on a blood vessel, in which laminar flow is assumed [Fig. 1(a)]. Light is backscattered and Doppler frequency shifted due to the movement of RBCs within the vessel. Two scattered beams, and , are selected with a dedicated pupil so that the angle between both beams is defined by the optical system and the length of the eye. The vector of the principal ray of each beam and the velocity of the blood in the vessel are all in the same plane. Assuming a quadratic velocity profile32 of the RBCs moving in the vessel, the power spectrum of the electronic signal has a theoretical step shape with a sharp edge at the maximum frequency shift (see spectra of Fig. 2). The maximum frequency shift corresponds to the maximum velocity of the blood in the center of the vessel. In this case, it can be shown that where and are the maximum frequency shifts of beams and , respectively, is the wavelength of the laser source, and is the refractive index of the plasma surrounding the RBCs. The angle , between the two scattered beams, is calculated using the formula , where represents the distance between the beam position ( and , Fig. 1) at the eye pupil and is the axial eye length assumed to be 23.95 mm33 and is set identical for all subjects. By observing the line of the probing beam on the fundus image acquired by the rtx1 camera, the angle [Fig. 1(a)] is adjusted to zero by rotating a Dove prism (DP) [Fig. 1(b)] so that it has no influence on the calculation of . In practice, an angle up to 10 deg (inducing an error of 1.5%) is acceptable to obtain a reliable measurement of .2.1.3.Optical layout of the velocimeterThe LDV optical system is introduced inside the illumination path with the beam splitter (BS) [Fig. 1(b)] and is located outside the adaptive optical path of the rtx1. A 830-nm laser source focused at infinity enters the optical system close to the edge of the pupil of the instrument, passes through the LDV and rtx1 optical systems to enter the pupil of the subject, and focuses on a vessel. The probing beam diameter on the retina is about . Part of this light is backscattered by the vessel and the RBCs and goes back through the pupil of the eye to the same optical system up to the image of the pupil plane where two holes select the two specific directions and . A small reflecting prism deflects each beam to lenses and , which focus each beam on a pinhole at the image plane and corresponding to about at the retinal plane. Detectors and (APD C5460, Hamamatsu) are placed just behind the pinholes. A diffractive element (DOE) placed at converts the spot in the fundus into a line. Then the (DP) is rotated to visually align this line with the vessel on the screen of the rtx1 (minimizing ). Lenses and form a telescope that relays the rtx1 pupil to the pupils of the LDV optical system with a unity magnification. 2.1.4.Signal acquisition and electronicsThe electrical signals from the two detectors are connected to an acquisition card (USB-6356, National Instruments) and are sampled at 120 kHz and Fourier analyzed. The direct current corresponding to the light intensity of a collected scattered light beam was removed by an analog filtering before the acquisition. During each of the 40 acquisitions of 100 ms, 45 ms are used to acquire one high-resolution image (rtx1), 30 ms for one pair of Doppler spectra (LDV) and the rest for the closed-loop AO. The trigger button of the rtx1 simultaneously starts recording the images on the rtx1 and the Doppler acquisition, which is controlled by a dedicated LabVIEW software based on a version developed for a laser Doppler flowmeter.34 2.1.5.Signal analysisThe power spectrum is given by , where . Considering the ideal case of a rectangular distribution of the power spectrum, the normalized function of partial summation: will linearly increase up to the maximum power spectrum and then remain constant (Fig. 2 dashed lines). First, the horizontal line is found with a linear fit of the last of the , for which only noise is expected. Then a second linear fit is done from to , corresponding to a 10% change between the first linear fit and the function. The intersection of both fitted lines is assumed to be the maximum frequency shift of the spectrum.2.1.6.Image analysisThe measurement of the vessel diameter close to the probing spot was implemented in MATLAB. Images are 16-bit gray scale with a resolution of , corresponding to roughly per pixel at the fundus. First, a threshold is applied at 80% of the maximal image intensity, and the probing beam spot is detected as the largest connected component. Then a square region of interest is set around the spot centroid, and a second-order polynomial is fitted to the intensity data to model the low-frequency variations due, among others, to the non-uniform illumination. The subsequent processing is applied on the difference between the original intensity values and the fitted model. The vessel orientation and the offset of its centerline with respect to the spot centroid are found by correlation with 24 precomputed synthetic vessel templates (15 deg increment). Finally, with this rough vessel orientation, image profiles are extracted across the vessel centerline with an interprofile distance of two pixels. The distance in pixels between the vessel edges is determined for each profile. As last step, the vessel diameter is estimated as the mean value of the non-aberrant edge distances determined on each profile (see Fig. 3). The conversion from pixels to physical distance is based on the publication of Bennet35 and was provided by Imagine Eyes©: where is the axial length in millimeters of the eye under examination.2.1.7.Calculation of mean flow rateAssuming a Poiseuille flow in a cylindrical cross section, the mean flow rate was calculated using the following equation: where is the diameter of the vessel obtained from the image analysis and is the maximum velocity of the RBCs in the vessel, which is derived from Eq. (1). The blood flow rate deviates by about 4% per 1 mm change in eye length.2.1.8.Measurement procedureThe subject was seated in front of the rtx1 and looked inside the rtx1 objective. A high-resolution image of the retina was displayed by the rtx1 to observe the selected vessel and the LDV probing beam. To observe retinal vessels close to the optic nerve head or outside the posterior pole, the range of movement of the internal rtx1 fixation point was not large enough, and therefore, the left eye was fixating on an external point, positioned 1.5 m behind the rtx1. Once the probing beam was moved on a first- or second-order retinal vessel in the temporal part of the fundus, the DOE was pushed into the optical path, converting the point into a line, which was then aligned with the retinal vessel by rotating the DP. Finally, the DOE was pulled out. The signals from the detectors were also connected to a loud speaker, so cardiac pulsed sound could be heard if the beam was focused on a retinal artery. The longitudinal beam position on the retinal vessel was optimized by varying the imaging focus plane until the spectra showed a clear cutoff (see Sec. 4) and the acoustic signal corresponded to a Doppler sound. The lateral position of the beam was adjusted with the external fixation point within about . 2.1.9.In Vitro measurements on latex microspheres flowing in a glass capillaryWe connected a glass capillary (drummond microcaps, inner diameter ) via a plastic tube to a syringe pump (Agilia SP, Fresenius Kabi, USA). Latex microspheres ( in diameter, Polysciences Europe GmbH, Eppelheim, Germany) suspended in water ( of microsphere in 35 mL of water) were pushed through the capillary with the syringe. Further, a focusing lens (, LB1761-B, Thorlabs) was mounted to focus the collimated probing beam on the capillary. This capillary-lens assembly was placed in front of the rtx1 objective. The vessel-simulating capillary was displayed on the rtx1 screen. Linearity was asssed by comparing the Doppler measurement of the maximum velocity of microspheres with the precise flow set by the syringe pump. 2.1.10.In Vivo measurements on venous junctionsThe study was conducted in accordance with the declaration of Helsinki for research involving human subjects and adhered to Good Clinical Practice guidelines. Written informed consent was obtained from the subjects after explaning the study. The study protocol was approved by the local Institutional Review Board (IRB #6705). For this exploratory study, no optimal sample was calculated, but six healthy subjects with a very good fixation ability, between the age of 27 and 58, participated in this study. A complete ocular examination, including slit lamp biomicroscopy, indirect funduscopy, fundus photography, and axial length measurement partial coherence interferometry (Zeiss IOL Master, Carl Zeiss Meditec Inc, Dublin, CA, USA), was conducted before the beginning of the study. Velocity measurements were conducted after dilatation of the right eye using a 1% topical tropicamide (Théa, Clermont-Ferrand, France). The rtx1 allows for an easy superposition of the pupil of the eye with that of the instrument. To test the accuracy of the LDV instrument, blood flow in a retinal venous junction of each human volunteers was measured before the junction and in the two daughter vessels. The measurement before the junction was compared with the sum of the measurements of the daughter vessels to assert their equality. An exemple of a vein and its daughter veins is illustrated in Fig. 4. 3.Results3.1.In Vitro Measurements on Latex Microspheres Flowing in a Glass CapillaryThe Doppler measurements performed on the glass capillary were found to be between 0.92 and for the velocity and between 1.75 and for the flow rate. The measurements were highly correlated (slope equals 1.09, , , see Fig. 5) with the flow imposed by the syringe pump. 3.2.In Vivo Measurements on Venous JunctionsMeasured diameters of the retinal veins varied between 89 and (mean ) (Table 1). The velocity ranged from 2.3 to (mean ). The blood flow values in the daughter and parent vessels ranged from 0.9 to (mean ). The standard deviation of was 18.5%. Table 1Measurements of RBF at retinal venous bifurcation in healthy subjects. ΔF is the difference between the flow in the parent vessel (FPV) and the sum of the flow in the daughter vessels (FDV1 and FDV2): ΔF=FPV−(FDV1+FDV2). Analysis was done automatically. Flow units are μL/min.
4.DiscussionThe development of this new prototype combining LDV and high-resolution AO imaging showed that (1) the LDV could be added using a complementary optical pathway; (2) optoelectronic development allowed for synchronization between both devices and therefore allowed for AO images and Doppler spectrum acquisitions every 0.1 s; (3) experimental measurements using glass capillary and microspheres showed high correlations between measurements and imposed values; and (4) preliminary measurements in human retinal vessels showed results with acceptable errors. 4.1.In Vitro Measurements on Latex Microspheres Flowing in a Glass CapillaryTo mimic a human retinal vessel, a glass capillary with a lens of 25.4 mm focal length was used. Capillaries in air with larger diameters would be preferable because they disturb the AO of the rtx1 less than smaller diameters. Our results show a clear linear relationship between the flow calculated with the Doppler measurements and the flow rate given by the syringe pump. 4.2.In Vivo Measurements on Venous JunctionsOur measurements of RBF were in the same range as those reported in previous studies of retinal vessels with similar diameters. Using a device based on the same fundamental principle, a bidirectional laser Doppler velocimeter, and monochromatic fundus photographs, Riva et al.16 measured retinal vessel diameters between 64 and , corresponding to flow rate between 1 and , which is consistent with our measurements. Yoshida et al.36 used a canon laser blood flowmeter (CLBF100) and measured the blood flow rate to be between 3.2 and (mean ) in retinal veins with a diameter ranging from 98 to (mean, ) (see Table 2). In their study, Werkmeister et al.18 measured retinal veins between 84 and and found RBF between 1.9 and with dual-beam bidirectional Doppler Fourier-domain optical coherence tomography. Table 2Comparaison between our measurements and the results from different publications.
4.3.aoLDV Advantages and FeasibilityaoLDV offers the possibility to perform very precise measurements of the inner diameter of vessels. Averaging 40 raw images, obtained from one measurement with the rtx1, each with a lateral pixel-resolution of ,39 gives a better lateral pixel-resolution of .40 In this study, raw images were used to calculate the diameter since each image corresponds to a pair of Doppler spectra. In our study, 94 out of 480 raw images (19.6%) were excluded from analysis, 31 (6.5%) because they could not be analyzed (mainly due to blinks) and 63 (13.1%) because the probing beam was not on the vessel. At the present time, no method to quantitatively assess perfusion is used in routine practice. This is due to the difficulties in performing RBF in vivo. The evaluation of a new method of RBF measurement is difficult in the absence of a gold standard. An LDV system was implemented by canon (CLBF100),36 but only a few instruments were sold. Nevertheless, LDV has not reached routine clinical practice, in part because of alignement difficulties and because of the relative imprecision of diameter measurements.41 The spatial resolution is limited by the quality of the eye under observation. OCT scanning is becoming standard in clinical optical imaging.42 OCT has a lateral resolution of around 12 to and an axial resolution between 2.3 and . The price of such cameras is about five times higher than classical fundus cameras, but they offer many advantages of which the most interesting is its ability to build axial slices of the fundus. By adapting the detection, OCT allows for Doppler measurement. However, the speed of the scanning limits the range of measurable velocities. Our proposed system encompasses this shortcoming since we do not scan the retina. 4.4.aoLDV LimitationsWe have encountered some limitations for the measurements of on human subjects, including the need for a good ocular fixation for 4 s to ensure that the probing and detection beams, respectively, hit and come back from the center of the vessel (Fig. 6). Owing to slight eye movements, we may underestimate by measuring velocities of RBCs close to the vessel wall (lower than ) or by adding Doppler shifts associated with the surrounding tissue. The AO live video of the fundus allowed us to verify the exact location of the laser beam on the vessel, but it did not provide the detection plane of the aoLDV, which could be different due to the AO adaptation. Bidirectional LDV measurement accuracy strongly depends on the plane of focus (conjugate to the detection plane) of the probing beam as well as A and B beams (Fig. 6) and the position of the probing beam, which should be exactly on the vessel center. This causes a parallax problem, which occurs when the image plane of the LDV is not on the same image plane as the rtx1 (Fig. 6). Obtaining a usefull pair of good spectra was a strong technical limitation. The constraint of the existing fundus camera rtx1 prevents us from using the path of the AO for the LDV, leaving this path uncorrected for eye aberrations. To overcome this parallax problem, we manually change the refraction correction of the rtx1 between and 3 diopters to find the best correction to match the detection plane. These settings were time-consuming, 1 h for one measurement of a bifurcation. The power spectrum from a glass capillary with a flowing fluid is typically a step function, in which the maximum of the frequency shift correponds to (cutt-off frequency). The quality of the in vitro spectrum obtained with the moving light scattering microspheres was excellent, and the cutoff frequency was always well-defined. Another difficulty was calculating the precise cutoff frequency of the in vivo spectrum, which, most of the time, did not have a clear step shape. Thus extraction was dependent on the algorithm to calculate the maximum of the frequency shift. 4.5.Clinical and Technical PerspectivesMore studies with a larger series of subjects are needed to evaluate the reproducibility and to estimate the total RBF after measuring the main retinal vessels near the optic nerve disc. To allow for examination of both eyes with the rtx1, an external fixation target should be designed. A more robust image analysis with deep learning would reject fewer images. The development of software combining data from the Doppler spectra, the quality of AO images, and the exact location of the probing beam on the retinal vessel is in progress and will allow for real-time evaluation of measurements. Assuming a correct focus, two illuminating beams and instead of collected scattered beams must hit at the fovea the same point as the probing beam (Fig 6). This would solve the parallax issue. 5.ConclusionIn conclusion, this aoLDV prototype allows for simultaneous measurement of the inner retinal diameter and the velocity of the RBC within that vessel, which allows for real-time calculation of absolute blood flow rate. The main limitations of this prototype were due to optomechanical constraints, which prevents the use of the optical path of AO for the LDV. This produced occasional discrepancies between the focal planes of the AO and LDV optical paths (parallax effect), which suppressed Doppler signal in one or both channels. Improving the analysis method and solving the parallax issue will offer a high potential for examining RBF abnormalities in patients with ocular or systemic diseases. The results obtained in healthy subjects are promising. DisclosuresFrederic Truffer, Martial Geiser, Helene Strese, and Christophe Chiquet are inventors of the patent FR3042963A1 and US10561314B2 (Dispositif de mesure de la vitesse du sang dans un vaisseau sanguin de l’oeil. Device for measuring the blood velocity in a blood vessel of the eye). This research was funded in part by Novartis. There are no other patents or products in development to declare. None of the authors have a competing interest even if some of the authors are inventors of the patent FR3042963A1 (Dispositif de mesure de la vitesse du sang dans un vaisseau sanguin de l’oeil. Device for measuring the blood velocity in a blood vessel of the eye). AcknowledgmentsNovartis Pharma S.A.S. (France) provided research funding for the preclinical part of this study. Association for research and teaching in Ophthalmology, Grenoble, France (ARFO AOLDV_2017) and University of Applied Sciences and Arts of Western Switzerland provided research and exchanges funding (No. 75678). Grenoble Alpes University Hospital, DRCI provided funding for human experiments (AOLDV 14.175). HES-SO gave partial salary to Frederic Truffer, Helene Strese, Gilbert Maître, and Serge Amoos. All other authors received no specific funding for this work. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. There are not patents or products in development to declare. All authors thanks Charles Riva for many discussions and helpful remarks. ReferencesC. J. Pournaras et al.,
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BiographyFrederic Truffer has a background in electrical and optical systems engineering and has worked for more than 13 years in the development of ophthalmology instruments. His focus is on laser Doppler flowmeters for human and small animals and he is strongly involved in projects with an absolute laser Doppler velocimeter. Martial Geiser, physicist, has more than 25 years of experience in lens design and optical instrumentation with numerous publications. His research is focused on optical instrumentation such as laser Doppler flowmetry or velocimetry, which has led to not only the recent development of compact flowmeters but also fluorescence sensing for microfluidic systems and multispectral imaging of the skin. Marc-Antoine Chappelet, fellow in ophthalmology, was strongly involved in the human clinical use of AO-LDV, in the clinical research team of Grenoble-Alpes University Hospital. His experience in retinal retina allowed him to successfully schedule the first measurements in humans and obtained a master of science. Helene Strese received a Bachelor of Engineering in optics from the University of Applied Sciences of Jena in Germany in 2013 and a Master of Science from the University of Applied Sciences of Western Switzerland in 2017. She specialized in lens design. Gilbert Maître received his MSc degree in electrical engineering from Swiss Federal Institute of Technology Zurich and his PhD from the University of Neuchâtel in 1994. Since 2005, he has been a research associate at the University of Applied Sciences of Western Switzerland, Sion. His main research interests are signal and image processing, as well as pattern recognition and machine learning. Serge Amoos is a senior scientist at the HES-SO. He has been working for several decades in the field of mechanical engineering, particularly in the development of industrial or laboratory equipment, as well as in the design of optical and medical devices. His skills range from preparation of specifications to rapid prototyping, manufacturing, and industrialization. Florent Aptel is a specialist of glaucoma at the University Hospital of Grenoble-Alpes and Department of Ophthalmology. He is highly involved in many projects in glaucoma from diagnosis, physiopathology, intraocular pressure measurements, treatment, and ocular blood flow. Christophe Chiquet is the head of the Department of Ophthalmology and Visual Sciences at the University Hospital of Grenoble-Alpes. His main interests include medical and surgical retina and uveitis. He developed a multimodal approach for the study of ocular blood flow, using laser Doppler velocimetry, adaptive optics, OCTA imaging, and computerized analysis of retinal vessels measurements. |