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17 August 2017 Epi-detecting label-free multimodal imaging platform using a compact diode-pumped femtosecond solid-state laser
Marco Andreana, Tuan Le, Anders K. Hansen, Aart J. Verhoef, Ole B. Jensen, Peter E. Andersen, Paul Slezak, Wolfgang Drexler, Alma Fernández, Angelika Unterhuber
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Journal of Biomedical Optics, Vol. 22, Issue 9, 091517 (August 2017). https://doi.org/10.1117/1.JBO.22.9.091517
Abstract
We have developed an epi-detected multimodal nonlinear optical microscopy platform based on a compact and cost-effective laser source featuring simultaneous acquisition of signals arising from hyperspectral coherent anti-Stokes Raman scattering (CARS), two-photon fluorescence, and second harmonic generation. The laser source is based on an approach using a frequency-doubled distributed Bragg reflector-tapered diode laser to pump a femtosecond Ti:sapphire laser. The operational parameters of the laser source are set to the optimum trade-off between the spectral and temporal requirements for these three modalities, achieving sufficient spectral resolution for CARS in the lipid region. The experimental results on a biological tissue reveal that the combination of the epi-detection scheme and the use of a compact diode-pumped femtosecond solid-state laser in the nonlinear optical microscope is promising for biomedical applications in a clinical environment.
CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
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Marco Andreana, Tuan Le, Anders K. Hansen, Aart J. Verhoef, Ole B. Jensen, Peter E. Andersen, Paul Slezak, Wolfgang Drexler, Alma Fernández, and Angelika Unterhuber "Epi-detecting label-free multimodal imaging platform using a compact diode-pumped femtosecond solid-state laser," Journal of Biomedical Optics 22(9), 091517 (17 August 2017). https://doi.org/10.1117/1.JBO.22.9.091517
Received: 2 December 2016; Accepted: 30 June 2017; Published: 17 August 2017
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Cited by 7 scholarly publications.
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KEYWORDS
Femtosecond phenomena
Second-harmonic generation
Semiconductor lasers
Sapphire lasers
Mirrors
Multimodal imaging
Diode pumped solid state lasers
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