Portable fluorescence lifetime spectroscopy system for in-situ interrogation of biological tissues

Marcelo Saito Nogueira; Alessandro Cosci; Ramon Gabriel Teixeira Rosa; Ana Gabriela Sálvio M.D.; Sebastião Pratavieira; Cristina Kurachi D.D.S.

doi:10.1117/1.JBO.22.12.121608

19 October 2017 Portable fluorescence lifetime spectroscopy system for in-situ interrogation of biological tissues

Marcelo Saito Nogueira, Alessandro Cosci, Ramon Gabriel Teixeira Rosa, Ana Gabriela Sálvio M.D., Sebastião Pratavieira, Cristina Kurachi D.D.S.

Author Affiliations +

Journal of Biomedical Optics, Vol. 22, Issue 12, 121608 (October 2017). https://doi.org/10.1117/1.JBO.22.12.121608

Abstract

Fluorescence spectroscopy and lifetime techniques are potential methods for optical diagnosis and characterization of biological tissues with an in-situ, fast, and noninvasive interrogation. Several diseases may be diagnosed due to differences in the fluorescence spectra of targeted fluorophores, when, these spectra are similar, considering steady-state fluorescence, others may be detected by monitoring their fluorescence lifetime. Despite this complementarity, most of the current fluorescence lifetime systems are not robust and portable, and not being feasible for clinical applications. We describe the assembly of a fluorescence lifetime spectroscopy system in a suitcase, its characterization, and validation with clinical measurements of skin lesions. The assembled system is all encased and robust, maintaining its mechanical, electrical, and optical stability during transportation, and is feasible for clinical measurements. The instrument response function measured was about 300 ps, and the system is properly calibrated. At the clinical study, the system showed to be reliable, and the achieved spectroscopy results support its potential use as an auxiliary tool for skin diagnostics.

Citation Download Citation

Marcelo Saito Nogueira, Alessandro Cosci, Ramon Gabriel Teixeira Rosa, Ana Gabriela Sálvio M.D., Sebastião Pratavieira, and Cristina Kurachi D.D.S. "Portable fluorescence lifetime spectroscopy system for in-situ interrogation of biological tissues," Journal of Biomedical Optics 22(12), 121608 (19 October 2017). https://doi.org/10.1117/1.JBO.22.12.121608

Received: 31 May 2017; Accepted: 22 September 2017; Published: 19 October 2017

ACCESS THE FULL ARTICLE

JOURNAL ARTICLE
10 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY