Division of focal plane polarimeter-based 3 × 4 Mueller matrix microscope: a potential tool for quick diagnosis of human carcinoma tissues

Jintao Chang; Honghui He; Ye Wang; Yi Huang; Xianpeng Li; Chao He; Ran Liao; Nan Zeng; Shaoxiong Liu; Hui Ma

doi:10.1117/1.JBO.21.5.056002

9 May 2016 Division of focal plane polarimeter-based 3 × 4 Mueller matrix microscope: a potential tool for quick diagnosis of human carcinoma tissues

Jintao Chang, Honghui He, Ye Wang, Yi Huang, Xianpeng Li, Chao He, Ran Liao, Nan Zeng, Shaoxiong Liu, Hui Ma

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Journal of Biomedical Optics, Vol. 21, Issue 5, 056002 (May 2016). https://doi.org/10.1117/1.JBO.21.5.056002

Abstract

A polarization microscope is a useful tool to reveal the optical anisotropic nature of a specimen and can provide abundant microstructural information about samples. We present a division of focal plane (DoFP) polarimeter-based polarization microscope capable of simultaneously measuring both the Stokes vector and the 3×4 Mueller matrix with an optimal polarization illumination scheme. The Mueller matrix images of unstained human carcinoma tissue slices show that the m₂₄ and m₃₄ elements can provide important information for pathological observations. The characteristic features of the m₂₄ and m₃₄ elements can be enhanced by polarization staining under illumination by a circularly polarized light. Hence, combined with a graphics processing unit acceleration algorithm, the DoFP polarization microscope is capable of real-time polarization imaging for potential quick clinical diagnoses of both standard and frozen slices of human carcinoma tissues.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Jintao Chang, Honghui He, Ye Wang, Yi Huang, Xianpeng Li, Chao He, Ran Liao, Nan Zeng, Shaoxiong Liu, and Hui Ma "Division of focal plane polarimeter-based 3 × 4 Mueller matrix microscope: a potential tool for quick diagnosis of human carcinoma tissues," Journal of Biomedical Optics 21(5), 056002 (9 May 2016). https://doi.org/10.1117/1.JBO.21.5.056002

Published: 9 May 2016

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