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3 June 2013 Video-rate imaging of microcirculation with single-exposure oblique back-illumination microscopy
Tim N. Ford, Jerome Mertz
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Journal of Biomedical Optics, Vol. 18, Issue 6, 066007 (June 2013). https://doi.org/10.1117/1.JBO.18.6.066007
Abstract
Oblique back-illumination microscopy (OBM) is a new technique for simultaneous, independent measurements of phase gradients and absorption in thick scattering tissues based on widefield imaging. To date, OBM has been used with sequential camera exposures, which reduces temporal resolution, and can produce motion artifacts in dynamic samples. Here, a variation of OBM that allows single-exposure operation with wavelength multiplexing and image splitting with a Wollaston prism is introduced. Asymmetric anamorphic distortion induced by the prism is characterized and corrected in real time using a graphics-processing unit. To demonstrate the capacity of single-exposure OBM to perform artifact-free imaging of blood flow, video-rate movies of microcirculation in ovo in the chorioallantoic membrane of the developing chick are presented. Imaging is performed with a high-resolution rigid Hopkins lens suitable for endoscopy.
© 2013 Society of Photo-Optical Instrumentation Engineers (SPIE) 0091-3286/2013/$25.00 © 2013 SPIE
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Tim N. Ford and Jerome Mertz "Video-rate imaging of microcirculation with single-exposure oblique back-illumination microscopy," Journal of Biomedical Optics 18(6), 066007 (3 June 2013). https://doi.org/10.1117/1.JBO.18.6.066007
Published: 3 June 2013
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Cited by 18 scholarly publications and 2 patents.
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KEYWORDS
Prisms
Capillaries
Tissues
Absorption
Blood circulation
Distortion
Video
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