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7 January 2013 Stepwise multiphoton activation fluorescence reveals a new method of melanin detection
Zhenhua Lai, Josef Kerimo, Yair J. Mega, Charles A. DiMarzio
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Journal of Biomedical Optics, Vol. 18, Issue 6, 061225 (January 2013). https://doi.org/10.1117/1.JBO.18.6.061225
Abstract
The stepwise multiphoton activated fluorescence (SMPAF) of melanin, activated by a continuous-wave mode near infrared (NIR) laser, reveals a broad spectrum extending from the visible spectra to the NIR and has potential application for a low-cost, reliable method of detecting melanin. SMPAF images of melanin in mouse hair and skin are compared with conventional multiphoton fluorescence microscopy and confocal reflectance microscopy (CRM). By combining CRM with SMPAF, we can locate melanin reliably. However, we have the added benefit of eliminating background interference from other components inside mouse hair and skin. The melanin SMPAF signal from the mouse hair is a mixture of a two-photon process and a third-order process. The melanin SMPAF emission spectrum is activated by a 1505.9-nm laser light, and the resulting spectrum has a peak at 960 nm. The discovery of the emission peak may lead to a more energy-efficient method of background-free melanin detection with less photo-bleaching.
© 2013 Society of Photo-Optical Instrumentation Engineers (SPIE) 0091-3286/2013/$25.00 © 2013 SPIE
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Zhenhua Lai, Josef Kerimo, Yair J. Mega, and Charles A. DiMarzio "Stepwise multiphoton activation fluorescence reveals a new method of melanin detection," Journal of Biomedical Optics 18(6), 061225 (7 January 2013). https://doi.org/10.1117/1.JBO.18.6.061225
Published: 7 January 2013
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Cited by 17 scholarly publications.
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KEYWORDS
Luminescence
Optical filters
Signal detection
Skin
Continuous wave operation
Pulsed laser operation
Image filtering
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