Laser guidance–based cell detection in a microfluidic biochip

Wan Qin; Lucas Schmidt; Xiaoqi Yang; Lina Wei; Ting Huang; Julie X. Yuan; Xiang Peng; Xiaocong Yuan; Bruce Z. Gao

doi:10.1117/1.JBO.18.6.060502

11 June 2013 Laser guidance–based cell detection in a microfluidic biochip

Wan Qin, Lucas Schmidt, Xiaoqi Yang, Lina Wei, Ting Huang, Julie X. Yuan, Xiang Peng, Xiaocong Yuan, Bruce Z. Gao

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Journal of Biomedical Optics, Vol. 18, Issue 6, 060502 (June 2013). https://doi.org/10.1117/1.JBO.18.6.060502

Abstract

We developed a microfluidic biochip to perform laser guidance on two cell types, chick embryonic forebrain neurons and spinal cord neurons. Observation of neurons under a high-magnification microscope, which we obtained from these two cell types, showed no difference in morphology. However, when flowing in the microfluidic channel and simultaneously being laser guided, the two cell types gained quite different guidance speeds under the same experimental conditions. The results demonstrate that different cell types with the same morphology (e.g., size, shape, etc.) can be effectively distinguished from each other by measuring the difference in guidance speeds (the maximum flow speed minus the initial flow speed). This technique is expected to provide a new approach to high-throughput, label-free cell sorting with high sensitivity.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Wan Qin, Lucas Schmidt, Xiaoqi Yang, Lina Wei, Ting Huang, Julie X. Yuan, Xiang Peng, Xiaocong Yuan, and Bruce Z. Gao "Laser guidance–based cell detection in a microfluidic biochip," Journal of Biomedical Optics 18(6), 060502 (11 June 2013). https://doi.org/10.1117/1.JBO.18.6.060502

Published: 11 June 2013

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