Investigation of tryptophan–NADH interactions in live human cells using three-photon fluorescence lifetime imaging and Förster resonance energy transfer microscopyn1

Vinod Jyothikumar; Yuansheng Sun; Ammasi Periasamy

doi:10.1117/1.JBO.18.6.060501

7 June 2013 Investigation of tryptophan–NADH interactions in live human cells using three-photon fluorescence lifetime imaging and Förster resonance energy transfer microscopyⁿ¹

Vinod Jyothikumar, Yuansheng Sun, Ammasi Periasamy

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Journal of Biomedical Optics, Vol. 18, Issue 6, 060501 (June 2013). https://doi.org/10.1117/1.JBO.18.6.060501

Abstract

A method to investigate the metabolic activity of intracellular tryptophan (TRP) and coenzyme-NADH using three-photon (3P) fluorescence lifetime imaging (FLIM) and Förster resonance energy transfer (FRET) is presented. Through systematic analysis of FLIM data from tumorigenic and nontumorigenic cells, a statistically significant decrease in the fluorescence lifetime of TRP was observed in response to the increase in protein-bound NADH as cells were treated with glucose. The results demonstrate the potential use of 3P-FLIM-FRET as a tool for label-free screening of the change in metabolic flux occurring in human diseases or other clinical conditions.

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Vinod Jyothikumar, Yuansheng Sun, and Ammasi Periasamy "Investigation of tryptophan–NADH interactions in live human cells using three-photon fluorescence lifetime imaging and Förster resonance energy transfer microscopyⁿ¹," Journal of Biomedical Optics 18(6), 060501 (7 June 2013). https://doi.org/10.1117/1.JBO.18.6.060501

Published: 7 June 2013

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