Imaging targeted-agent binding in vivo with two probes

Brian W. Pogue; Kimberley S. Samkoe; Shannon K. Hextrum; Julia A. O'Hara; Michael Jermyn; Subhadra Srinivasan; Tayyaba Hasan

doi:10.1117/1.3449109

1 May 2010 Imaging targeted-agent binding in vivo with two probes

Brian W. Pogue, Kimberley S. Samkoe, Shannon K. Hextrum, Julia A. O'Hara, Michael Jermyn, Subhadra Srinivasan, Tayyaba Hasan

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Journal of Biomedical Optics, Vol. 15, Issue 3, 030513 (May 2010). https://doi.org/10.1117/1.3449109

Abstract

An approach to quantitatively image targeted-agent binding rate in vivo is demonstrated with dual-probe injection of both targeted and nontargeted fluorescent dyes. Images of a binding rate constant are created that reveal lower than expected uptake of epidermal growth factor in an orthotopic xenograft pancreas tumor (2.3×10−5 s−1), as compared to the normal pancreas (3.4×10−5 s−1). This approach allows noninvasive assessment of tumor receptor targeting in vivo to determine the expected contrast, spatial localization, and efficacy in therapeutic agent delivery.

©(2010) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Brian W. Pogue, Kimberley S. Samkoe, Shannon K. Hextrum, Julia A. O'Hara, Michael Jermyn, Subhadra Srinivasan, and Tayyaba Hasan "Imaging targeted-agent binding in vivo with two probes," Journal of Biomedical Optics 15(3), 030513 (1 May 2010). https://doi.org/10.1117/1.3449109

Published: 1 May 2010

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