Reflective interferometric chamber for quantitative phase imaging of biological sample dynamics

Natan Tzvi Shaked; Yizheng Zhu; Nima Badie; Nenad Bursac; Adam P. Wax

doi:10.1117/1.3420179

1 May 2010 Reflective interferometric chamber for quantitative phase imaging of biological sample dynamics

Natan Tzvi Shaked, Yizheng Zhu, Nima Badie, Nenad Bursac, Adam P. Wax

Author Affiliations +

Journal of Biomedical Optics, Vol. 15, Issue 3, 030503 (May 2010). https://doi.org/10.1117/1.3420179

Abstract

We introduce a new interferometric setup for single-exposure wide-field holographic phase imaging of highly dynamic biological samples. In this setup, the interferometric signal originates from a specially designed reflective interferometric chamber (InCh), creating an off-axis interferogram on the output plane of the system. The setup only requires the InCh and a simple reflection-mode two lens imaging system, without the need for additional optical elements such as gratings in the beam path. In addition, due to the close-to-common-path geometry of the setup, phase noise is greatly reduced. We experimentally compare the inherent phase stability of the system in ambient conditions to that of a conventional interferometer. We also demonstrate use of this system for wide-field quantitative phase imaging of two different highly dynamic, optically transparent biological samples: beating myocardial cells and moving unicellular microorganisms.

©(2010) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Natan Tzvi Shaked, Yizheng Zhu, Nima Badie, Nenad Bursac, and Adam P. Wax "Reflective interferometric chamber for quantitative phase imaging of biological sample dynamics," Journal of Biomedical Optics 15(3), 030503 (1 May 2010). https://doi.org/10.1117/1.3420179

Published: 1 May 2010

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